EFFECT OF HONEY ON HEALTHY AND ALLOXAN DIABETES SWISS- WEBSTER MICE (Mus musculus) WITH AND WITHOUT GLIBENCLAMIDE THERAPY

• Main Author: Susbandiyah Ifada, Andy
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/45909
• Institution: Institut Teknologi Bandung

Diabetes mellitus and its complication are major causes of death in most countries. Some studies showed that diabetes can be treated by natural honey. Honey has been known to have many benefits such as antibacterial and anti-inflammatory. Nevertheless, the high content of fructose and glucose in honey should be taken into consideration in its use for people with diabetes mellitus. This study aims to know the effect of honey, especially honey from Sumbawa, West Nusa Tenggara in healthy mice and alloxan-induced diabetic mice. This study consists of honey quality test, a glucose tolerance test (GTT) and alloxan-induced diabetes. GTT was performed on healthy animals. Animals were given pure honey, diluted honey 20% and 50%, blood glucose levels were measured every 30 minutes for 3 hours. Alloxan diabetes test conducted on mice by inducing alloxan dose of 50 mg/kg bw. Animals with blood glucose levels of 400-500 mg/dL were used as diabetic animal in research. Honey and combination of glibenclamide and honey given for 21 days. Glibenclamide dose of 0.65 mg/kg bw used as standard drug. Blood glucose levels were measured on days 10, 17 and 24. On the next day, the animals were sacrificed and pancreas were isolated. The Pure, 20%, and 50% honey showed raise blood glucose levels. Blood glucose levels in mice that given pure honey stayed in the normal value of 140 mg/dL until 180 minute observation, significantly different from the group of glucose 20% (p<0.05). Honey and combination of glibenclamide and honey in alloxan diabetes test did not cause a decrease of blood glucose levels that significantly different compared to the sore group (p>0.05). Pancreatic histology results did not show an improvement in the condition of the islands of Langerhans after administration of honey, glibenclamide or a combination of glibenclamide and honey. Honey maintains blood glucose levels of healthy mice on a normal value of 140 mg/dL until 180 minutes. In alloxan diabetes test, combination of glibenclamide and honey did not show a decrease in blood glucose levels.