THE ANTIBACTERIA ACTIVITY OF ETHANOL EXTRACT OF POMEGRANATE PEEL (Punica granatum L.) AND LEMON GRASS (Cymbopogon citratus) AND ITS COMBINATION AGAINST KLEBSIELLA PNEUMONIAE, ESCHERICHIA COLI, STAPHYLOCOCCUS AUREUS AND PSEUDOMONAS AERUGINOSA

• Main Author: Ahda Novitri, Suci
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/45911
• Institution: Institut Teknologi Bandung

Diabetic ulcers are one of the chronic complications of diabetes mellitus in the form of an open wound on the surface of the skin that can be accompanied by local tissue death. Approximately 58% of patients were infected diabetic foot ulcers clinically. The purpose of this study was to find an alternative medicinal plants that have antibacterial activity for diabetic ulcer. This study were divided into two studies: the initial study and the experimental study. In the initial study, profile of bacteria in diabetic ulcer patients in a Hospital in Bandung during 2014 and sensitivity of antibiotics to the bacterias were collected. This initial study was retrospective study. Then in experimental study, activity of antibacteria of the test extracts with microdilution method and agar diffusion to determinte the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). Then the test of combination effect of the extracts was done using paper strip diffusion. The selected test plants were pomegranate peel and lemon grass. The results obtained in the initial study showed that the highest prevalence of bacteria in diabetic ulcer patients in a Hospital in Bandung during 2014 were K. pneumoniae (19,70%), E. coli (15,15%) and S. aureus (9,09%). P. aeruginosa was the most antibiotic resistency. Ciprofloxacin was the most resistant antibiotic against K. pneumonia, E. coli and P. aeruginosa. Then in experimental study, Klebsiella pneumoniae, Esherichia coli ATCC 8739, Staphylococcus aureus ATCC 6538 and Pseudomonas aeruginosa ATCC 9027 were chosen to test the antibacterial activity of the test plants. The results obtained in the experimental study showed that the ethanol extract of pomegranate peel and lemon grass showed antimicrobial activity against all the test bacterias with MIC of 256 µg/mL and 4096 µg/mL, respectively. Aditive interaction effect against S. aureus ATCC 6538, E. coli ATCC 8739 and P. aeruginosa ATCC 9027 were obtained from the combination of the both extracts. Synergistic interaction effect against K. pneumoniae was obtained from the combination of the both extracts. The conclusion of this study is the ethanol extract of pomegranate peel is potential as antimicrobial and its combination with the ethanol extract of the lemongrass shows synergistic interaction effect against K. pneumoniae.