PHARMACOKINETIC AND BIODISTRIBUTION STUDY OF PLGA- POLOXAMER LABELLING QUANTUM DOT FLUORESCENT NANOCARRIER IN MICE

• Main Author: Husni, Patihul
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/45932
• Institution: Institut Teknologi Bandung

Nanocarrier which has ability to circulate long term in the bloodstream is an important thing to improve interaction between nanoparticle and infected cell. PLGA Polymeric based nanocarrier has been used as an effective carrier for drug delivery into the cell. PLGA nanoparticles are easily recognized by the body immune system and then cleared from the circulation. PLGA nanoparticles has a a hydrophobic surface so that it can be recognized by opsonin and eliminated by macrophage (reticuloendothelial system/RES). Modification of PLGA nanoparticles surface with hydrophilic polymer such as poloxamer is used to avoid opsonization so that nanoparticles can circulate long term. In evaluation of long-circulating nanoparticle, it’s needed a long term stable and sensitive sensor. Fluorescent quantum dot can be used for the purpose. The aim of this study was to develop formula of long circulating PLGA-Poloxamer nanocarrier with the quantum dot as a sensor. Characterization, stability study, pharmacokinetic and biodistribution study were done to the nanoparticles. PLGA-Poloxamer nanoparticles were prepared by nanoprecipitation method and purified by dialysis. Characterization of nanoparticles was particle size, polydispersity index and potensial zeta. Stability study of nanoparticle in serum was performed to check the fluorescence stability of quantum dot under serum condition. The study results showed that the particle size of nanoparticles was between 90-150 nm with polydispersity index of approximately 0.2-0.4 and negative charge of potensial zeta. Encapsulation efficiency of quantum dot was more than 90%. Stability study showed that quantum dot fluorescent was relative stable for 48 h under serum condition. In the pharmacokinetic study, NP 5% and NP 10% showed longcirculating profile. The higher poloxamer concentration was used, the higher AUC value and the lower clearance value of nanoparticles were obtained. Based on the study results, it can be concluded that using of 5% and 10% poloxamer can provide steric barrier on nanoparticle and provide high concentration nanoparticle in the bloodstream with low accumulation in organ include RES organ.