INTERACTION BETWEEN CHOLINERGIC AND OPIOID SYSTEMS IN RELAPSE TO ALCOHOL DEPENDENCE

• Main Author: Sari Lebang, Julianri
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/45954
• Institution: Institut Teknologi Bandung

Background : Relapse in addiction is a common feature of drug addiction, including to alcohol. Exposure to addictive substances can cause relapse in individuals who are abstinent from the substance because of the similarity of neurotransmitter pathways in the brain that play role in dependence.The purposeofthis study todetermine alteration in cholinergicsystemduring relapse inalcohol dependence because of exposure to morphine which describesthe interaction of cholinergic-opioid system.Methods : Testing was conducted using Conditioned Place Preference(CPP) paradigm which consist of four phases testing: habituation, conditioning, abstinence and relapse. Habituation test was conducted to acclimatized the animals to apparatus CPP, room test and treatment. In conditioning test animals were given alcohol, nicotine and combination of both. Animals were fasted from drug in abstinent periods followed by priming dose of morphine for testing relapse. AChE activity measurements were performed at the end of test using Ellman’s method. Results: Primingdose of morphine of10mg/kg, 20mg/kgand40mg/kg BWincreased significantly thepreferencescore in relapse to alcohol comparedwith preference scorein postconditioningtest, whilea dose of5mg/kg BWshowed no significantdifferenceinpreferencescores.There were a significant differences between score after priming dose of 5 mg/kg BW and 20 mg/kg BW comparedto priming dose of 40 mg/kg BW.AChE activity in animals at relapse was significantly different compared to saline group. The highest enzyme activity was shown after priming dose 20 mg/kg BW. There was no significant difference between the activity of AChE in groups receiving 5 mg/kg, 10 mg/kg, 20 mg/kg and 40 mg/kg BW of morphine.Conclusion: This study showed that exposure to morphine increasepreference scores during relapse in animal with alcohol dependence, which was accompanied by increased AChE activity.This result suggest interaction between cholinergic-opioid system in alcohol dependence.