ACUTE AND SUBCHRONIC ORAL TOXICITY STUDIES OF ETHYL ACETATE EXTRACT OF SONCHUS ARVENSIS L. LEAVES
The plant Sonchus arvensis L., known in Indonesia as tempuyung, has been used widely as traditional drug. The most well-known activity of this plant is antikidney stone. Other uses of the plant include as diuretic agent, and amelioration of the impaired glucose tolerance. This plant has also been...
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| Format: | Theses |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/45977 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | The plant Sonchus arvensis L., known in Indonesia as tempuyung, has been used
widely as traditional drug. The most well-known activity of this plant is antikidney stone. Other uses of the plant include as diuretic agent, and amelioration of
the impaired glucose tolerance. This plant has also been reported to have
protective effect against CCl4-induced kidney damage in rat. Prior to human use,
it is mandatory that a traditional drug should meet the safety requirement.
Previous study showed that the ethyl acetate fraction of ethanol extract of Sonchus
arvensis L. gave the highest activity in xanthine oxidase inhibition. In the present
study acute oral toxicity and subchronic toxicity tests of ethyl acetate extract of
Sonchus arvensis L. leaves were carried out to explore its safety feature. Extract
was prepared by maceration in ethyl acetate. Acute oral toxicity test was
conducted in ddY mice using the conventional method. Sixty mice were divided
into 6 groups, namely control group and five test groups, each given the extract at
0.19 g/kg body weight (b.w), 0.56 g/kg b.w, 1.67 g/kg b.w, 5 g/kg b.w and 15
g/kg, respectively. Subchronic toxicity test was carried out in Wistar rats by daily
administraion of ethyl acetate extract of Sonchus arvensis L. leaves for 90 days at
100 mg/kg b.w, 400 mg/kg b.w, and 1000 mg/kg b.w. The rats in all groups were
observed for behaviour, body weight development, hematology, clinical
biochemistry, organ to body weight ratio, and organ histology. No mortality was
observed both in acute and subchronic toxicity tests in male and female animals.
Observed behaviour, body weight profile and organ histology among
experimental groups were comparable. Hematology, clinical biochemistry
parameters, organ to body weight ratio were not significantly different.
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