ANTIOXIDANT ACTIVITY O}- VARIOUS SHELLS EXTRACTS FROM FOUR SPECIES OF FABACEAE WITH DPPH AND FRAP ASSAYS
Background and obiectives: tr4a y things may halm health condition, such as lree radicals. lultheflrore antioxidants are needcd 1() neutralize liee rcdicals aclions. Many plants has anlioxidant aclivity including several nut and bean. Fabaceae family contained phenolic and flavonoid compounds. Th...
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| Format: | Theses |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/45997 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Background and obiectives: tr4a y things may halm health condition, such as lree
radicals. lultheflrore antioxidants are needcd 1() neutralize liee rcdicals aclions.
Many plants has anlioxidant aclivity including several nut and bean. Fabaceae
family contained phenolic and flavonoid compounds. The aim olthis research were
to study a tioxidant activitv of various shells extracts frorn lour species of
Fabaccac using lwo methods ol a tioxidant resliig DPPH (2-2-diphenyl-1
picryihydrazyl), FRAP (Ferric Reduclng Antioridant Power) and its corelation
with total phenolic, llavonoid and carotenoid cortent Nlethod: Extraction was
perlonDcd by reflux using gradient polarity sohelrt. Lach extract were obsened by
thin layer chromatography (TLC), andoxidant aclivity by DPPH,II{{P assays, lcjo
of DPPH scalenging activity, ECt0 of I'RAP capacity. detelminalion of rotal
phcnolic. IlavoDoid and carolenoid content rrere perlomcd by spectrophotometry
UV-Vis aftl its conclation with DPPII and FRAP capacities by Pearson meilod.
Result: The highest DPFH scaverging aclivily (75.97 %) was givcn by ethancl
sliells extmct ol peairut and the highesr FRAP capaciry (16-:15 %) 1l,as given by
heranc shells extmct of kidney beao. Ethanolic shclls extract ol peanut had lhc
highest phenolic oontcnt (6.91 g GAE/100 g) and ethyl acetalc shells extmct of
peanut had the highest total flavonoid (13.37 g QE/100 g). ethyl acetate shells
exlract oi soybean had the highest carotenoid 0.33 g BIjT,/100 g. Total phenolic of
aIl ofshelis exl.acts Lom tbur species ollabaceac (solbean. red kidney bean, bogor
peanut and peanut) had highly positive colrelation with DPPH scavenging activity (r
= 0.732, p<0.05. r = 0.985, p< 0.01. r:0.E4,+- p.:O.{li, r = 0.984, p<0.01)
Conclusion: All of extracts sample (except n hcxanc shelis exiract ol so_vbean)
could be olassilied as vcry slrong anlioxidant. Phenolic compoulrds were thc major
contributor in extracl offour species ofFabaceae in DPPII scarcnging activities.
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