PROFILING RHODESIELLA SP. GUT MICROBIOME WITH A METAGENOMICS APPROACH AS DETERMINING BANANA BLOOD DISEASE VECTOR
Rhodesiella manii and Rhodesiella bhutanensis are known to have the highest abundance in inflorescences of infected bananas with blood diseases. It is strongly suspected that the role of the insect Rhodesiella sp. as a vector of Banana Blood Disease (BBD) in the spread of Blood Disease Bacterial. It...
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id-itb.:462442020-02-25T13:49:10ZPROFILING RHODESIELLA SP. GUT MICROBIOME WITH A METAGENOMICS APPROACH AS DETERMINING BANANA BLOOD DISEASE VECTOR Yasman, Annisaulhusna Indonesia Theses Rhodesiella sp., Microbiome, Banana Blood Disease, Metagenomic INSTITUT TEKNOLOGI BANDUNG https://digilib.itb.ac.id/gdl/view/46244 Rhodesiella manii and Rhodesiella bhutanensis are known to have the highest abundance in inflorescences of infected bananas with blood diseases. It is strongly suspected that the role of the insect Rhodesiella sp. as a vector of Banana Blood Disease (BBD) in the spread of Blood Disease Bacterial. It is necessary to know in detail how the composition and structure of the microbial community inhabit the gut area of the insect Rhodesiella sp. The study was conducted by utilizing a next-generation sequencing platform from the 16S rRNA gene to identifying the gut microbiota community of Rhodesiella manii and Rhodesiella bhutanensis which were collected from banana plantations infected with BBD in Subang, West Java, Indonesia. These sequences obtained were analysed through the quality checking, ?-diversity, ?-diversity and differential abundance analysis using QIIME2 software, and identification of pathogenic bacteria causing BBD. A total of 205 bacterial Operational Taxonomy Units (OTU) were obtained and overall gut microbiota composition Rhodesiella sp. consists of bacterial phyla Proteobacteria (99%). The results of genus-level taxa analysis showed that the bacteria Serratia, Wolbachia and Bartonella had the highest abundance in all insect samples with an average percentage of about 24% each. Ralstonia bacterium has been known belonging to a pathogenic group that causes wilting disease in plants and closely related to the Blood Disease Bacterium which has an average abundance of 1.4% in all insect samples, thus it was concluded that Rhodesiella sp. potentially spread BBD. The results of this study support the information that Rhodesiella sp. acts as a vector insect and uses as a reference for future BBD disease control by utilizing the composition of insect microbiota. text |
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Rhodesiella manii and Rhodesiella bhutanensis are known to have the highest abundance in inflorescences of infected bananas with blood diseases. It is strongly suspected that the role of the insect Rhodesiella sp. as a vector of Banana Blood Disease (BBD) in the spread of Blood Disease Bacterial. It is necessary to know in detail how the composition and structure of the microbial community inhabit the gut area of the insect Rhodesiella sp. The study was conducted by utilizing a next-generation sequencing platform from the 16S rRNA gene to identifying the gut microbiota community of Rhodesiella manii and Rhodesiella bhutanensis which were collected from banana plantations infected with BBD in Subang, West Java, Indonesia. These sequences obtained were analysed through the quality checking, ?-diversity, ?-diversity and differential abundance analysis using QIIME2 software, and identification of pathogenic bacteria causing BBD. A total of 205 bacterial Operational Taxonomy Units (OTU) were obtained and overall gut microbiota composition Rhodesiella sp. consists of bacterial phyla Proteobacteria (99%). The results of genus-level taxa analysis showed that the bacteria Serratia, Wolbachia and Bartonella had the highest abundance in all insect samples with an average percentage of about 24% each. Ralstonia bacterium has been known belonging to a pathogenic group that causes wilting disease in plants and closely related to the Blood Disease Bacterium which has an average abundance of 1.4% in all insect samples, thus it was concluded that Rhodesiella sp. potentially spread BBD. The results of this study support the information that Rhodesiella sp. acts as a vector insect and uses as a reference for future BBD disease control by utilizing the composition of insect microbiota.
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| format |
Theses |
| author |
Yasman, Annisaulhusna |
| spellingShingle |
Yasman, Annisaulhusna PROFILING RHODESIELLA SP. GUT MICROBIOME WITH A METAGENOMICS APPROACH AS DETERMINING BANANA BLOOD DISEASE VECTOR |
| author_facet |
Yasman, Annisaulhusna |
| author_sort |
Yasman, Annisaulhusna |
| title |
PROFILING RHODESIELLA SP. GUT MICROBIOME WITH A METAGENOMICS APPROACH AS DETERMINING BANANA BLOOD DISEASE VECTOR |
| title_short |
PROFILING RHODESIELLA SP. GUT MICROBIOME WITH A METAGENOMICS APPROACH AS DETERMINING BANANA BLOOD DISEASE VECTOR |
| title_full |
PROFILING RHODESIELLA SP. GUT MICROBIOME WITH A METAGENOMICS APPROACH AS DETERMINING BANANA BLOOD DISEASE VECTOR |
| title_fullStr |
PROFILING RHODESIELLA SP. GUT MICROBIOME WITH A METAGENOMICS APPROACH AS DETERMINING BANANA BLOOD DISEASE VECTOR |
| title_full_unstemmed |
PROFILING RHODESIELLA SP. GUT MICROBIOME WITH A METAGENOMICS APPROACH AS DETERMINING BANANA BLOOD DISEASE VECTOR |
| title_sort |
profiling rhodesiella sp. gut microbiome with a metagenomics approach as determining banana blood disease vector |
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https://digilib.itb.ac.id/gdl/view/46244 |
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