MICROALGAE LIPID INDUCTION TECHNIQUES THROUGH ADDITION DIFFERENT SOURCES AND CONCENTRATIONS OF NITROGEN

• Main Author: Hanif, Fadhilah
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/46246
• Institution: Institut Teknologi Bandung

Microalgae has potential as a raw material for producing biodiesel because microalgae contain lipids which can be further processed to be biodiesel, one of which is Chlorella vulgaris. However, the lipid content is not optimal, so lipid induction techniques need to be developed and a high lipid concentration can be obtained. Therefore, the aim of this study is the development of lipid induction techniques from Chlorella vulgaris microalgae to produce high lipid concentrations with high fatty acid profile. Chlorella vulgaris culture was cultivated on benneck media with urea added each at 10 g/L, 20 g/L, and 30 g/L (containing nitrogen respectively 4,752 g/L; 9,382 g/L; and 14.082 g/L) and KNO3 each at 35 g/L, 70 g/L, and 105 g/L (containing nitrogen respectively 4,932 g/L; 9,782 g/L; and 14,632 g/L). Then the harvesters are carried out from day 1 to day 16. At the time of harvesting, biomass was obtained and lipid extraction was measured using the bligh dyer method. Then an analysis of fatty acid profiles was carried out using Gas Chromatography FID. The results showed the growth of Chlorella vulgaris was influenced by the source and concentration of nitrogen. In the source of urea and KNO3, the growth of Chlorella vulgaris increased with the increase in nitrogen concentration. However, the resulting lipids will increase along with a decrease in the concentration of nitrogen in both nitrogen sources. For the profile of fatty acids produced, the urea and KNO3 samples were categorized quite well and had sufficient potential to become biodiesel in which both samples contained palmitic acid, oleic acid, and linoleate acid with a composition in the urea sample each at 23.54% , 22.66%, and 8.91% and in KNO3 samples respectively 22.69%, 9.48%, and 15.26%. The conclusion of this study is the concentration of nitrogen using urea and KNO3 sources can produce higher lipid content