ANTIOXIDANT ACTIVITY OF FOUR ASTERACEAE PLANTS USING DPPH METHOD AND ISOLATION OF ACTIVE COMPOUND FROM SELECTED PLANT

• Main Author: Bayu Indradi, Raden
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/46549
• Institution: Institut Teknologi Bandung

Background and Objectives: Free radicals are atoms or molecules that have one or more unpaired electrons on its outer orbital, highly reactive, and could damage cell inside human body. Body of human produce antioxidant to neutralize free radicals, but it’s decreasing as human ageing and stress oxidative conditions could increase the formation of free radicals, therefore an exogenous antioxidant are needed. Plants are well known as source of antioxidants. Asteraceae family is the largest family among the plant kingdom and easy to find in Indonesia, therefore it has great potential as source of exogeneous antioxidants. The objectives of this research were to determine antioxidant activities of aerial part of elephant’s foot (Elephantopus scaber), false daisy (Eclipta prostrata), Indian pluchea (Pluchea indica), and dandelion (Taraxacum officinale) using DPPH method, determine total flavonoid and total phenolic content, analyze correlation between total flavonoid content and total phenolic content with antioxidant activity, and isolation of active compound from selected plant. Methods: Extraction was carried out by reflux with increasing polarity using n)hexane, ethyl acetate, and ethanol respectively. Antioxidant activity was determined by DPPH method. Total flavonoid content was determined using Chang’s method and total phenolic content was determined using Folin—Ciolacteau reagent. Correlation of total flavonoid and total phenolic content with their antioxidant activities were analyzed by Pearson’s method. Fractination of selected plant was performed by vacuum liquid chromatography (VLC) and monitored by thin layer chromatography (TLC). Subfractination was conducted by column chromatography, and the second subfractination was done using chromatotron. Purification was carried out by preparative TLC and monitored by single development TLC using 3 different polarity of mobile phases. Characterization of isolate was done by specific spray reagent. Result: Ethanolic extract of Indian pluchea showed the highest antioxidant activity with IC50 DPPH 16.66 ± 0.08 µg/mL. The highest total phenolic content (23.49 ± 0,56 g QE (Quercetin Equivalent)/100 g) wasgiven by ethyl acetate extract of Indian pluchea, while thehighest flavonoid content (16.48 ± 0.25 g GAE (Gallic Acid Equivalent)/100 g) was showed by ethanolic extract of Indian pluchea. Conclusion: Indian pluchea herbs extract had the highest antioxidant activity compared to elephant’s foot, false daisy and dandelion herbs, using DPPH method. Total phenolic of elephant’s foot, false daisy and Indian pluchea herbs extract showed significantly negative correlation with their IC50 of DPPH scavenging activities. Isolated antioxidant compound was supposed to be a triterpenoid compound.