GENETIC VARIABILITY LEVEL ANALYSIS OF AEDES AEGYPTI MOSQUITO FROM BANDUNG BY USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) METHOD

• Main Author: Anggrereini (NIM : 206 95509), Evita
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/4673
• Institution: Institut Teknologi Bandung

<b>Abstract :</b><p align="justify">Random Amplified Polymorphic DNA (RAPD) method has been used to identify the genetic variability of Aedes aegypti, a dengue vector from Bandung, West Java. The mosquitoes were collected from Sukajadi and Padasuka (endemic area), Cibiru and Tamblong (non endemic area), based on the data of dengue risk area from "Dinas Kesehatan Kotamadya Bandung". Six oligonucleotides were used as RAPD's primer. Ae. aegypti from NAMRU (Naval Medical Research Unit, USA/Department of Health RI) was used for comparison. The result of RAPD qualitative analysis showed that the individual polymorphism level of mosquitoes from Sukajadi, Padasuka, Cibiru and Tamblong were 74 %,80%, 72% and 66% respectively. Analysis of all DNA bands based on all areas in Bandung, indicates that polymorphism level of Ae. aegypti from Sukajadi (94.1%) and Padasuka (95.3%) areas were higher than Cibiru (94.1%) and Tamblong (94.0%). The results of these study indicated that oligonucleotides primer of OPE 19 gave a specific band for NAMRU mosquitoes with a size of 760 bp. The total yield of mosquitoes DNA band ranged from 317 to 26139 bp. RAPD quantitative analysis using all primer showed that Bandung mosquitoes formed a separate group from NAMRU mosquito. However Ae. aegypti population from Sukajadi, Padasuka and Cibiru did not showed a systematic grouping pattern and among the sampling area, the Tambiong mosquitoes formed another group and showed a higher similarit index.