EVALUASI PENINGKATAN PEROLEHAN MINYAK BUMI OLEH BAKTERI INDIGEN DARI SUMUR X, JAWA BARAT DENGAN SIMULASI MICROBIAL ENHANCED OIL RECOVERY (MEOR) MENGGUNAKAN SAND-PACK COLUMN

• Main Author: Zainiya Millati, Ghaida
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/46737
• Institution: Institut Teknologi Bandung

Nowadays, global population growth soar rapidly resulted in the highly of energy demands increased, one of the energies is crude oil. In contrast, the productivity of oil field reservoir exist are depleted due to the oil field maturity and only one third crude oil are capable to recovered from reservoir by conventional crude oil extraction technology. Therefore, it is important to find alternative technology to enhance oil recovery. Microbial enhanced oil recovery (MEOR) is a technology, which employ microorganisms to enhance oil recovery. Microorganisms are able to improve oil recovery by increasing their mobility and viscosity alteration. The objective of this paper was to select and screen potential thermophilic hydro-carbonoclastic bacteria and evaluate its capability on the enhanced oil recovery for MEOR application. Hence, thermophilic hydro-carbonoclastic bacteria were isolated in two-stage isolation from X oil reservoir, West Java and gradually adapted in high temperature. Afterwards, the bacteria were screened its capability on crude oil degradation by enzymatic activity test and analyzed saturated, aromatic, resin, and asphaltic (SARA) composition. Selection were continued to determined optimization of inoculum amount and composition on three different variations, 10% (v/v)); 12.5% (v/v); 15 %(v/v), based on SARA composition, viscosity, and bacteria growth. MEOR simulation was running on selected isolated using sand-pack column. Results showed that there were 50 in total bacteria obtained which consist of 40 isolates from first-stage isolation and 10 isolates from the second-stage isolation and morphology dominance observed was Bacilli. Screening on enzymatic activity test and SARA composition resulted in ABG2-7, degraded asphaltic (8.06%), and ABG2-47, degraded asphaltic (14.48%) and aromatics (9.29%), as selected isolate. Hydrocarbon degradation test using inoculum amount and composition variations of ABG2-7 as individual isolate and consortium of ABG2-7:47 showed that individual isolate with 15% (v/v) inoculum was highest in reduction of viscosity up to 4.19 cP, the degraded asphaltic fraction to 17.52%, 12 h exponential growth, and specific growth to 0.24/h. Identification of 16S rRNA gene encoded showed that ABG2-7 isolates was Bacillus licheniformis. MEOR simulation using sand-pack column by ABG2-7 (15 %(v/v)) isolate able to improve oil recovery to 1.29% compare to control (0%). To conclude, individual isolate of ABG2-7 (15 %(v/v)) are potential to be applied on MEOR technology.