STABILITY TEST OF BIOSURFACTANT FROM BACILLUS SP. AND PSEUDOXANTHOMONAS SP. AND ITS APPLICATION IN MICROBIAL ENHANCED OIL RECOVERY (MEOR) USING SAND PACK COLUMN METHOD

• Main Author: Firmansyah, Yoga
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/46845
• Institution: Institut Teknologi Bandung

Has been developed Microbial Enhanced Oil Recovery (MEOR) technology to optimize the acquisition of petroleum from remaining reserves. MEOR application can be done by directly injected microorganisms or bioproducts into the reservoir of petroleum wells. One of those bioproducts is biosurfactant, which plays a role in lowering the surface tension between oil and water. Bacillus sp. F1 and Pseudoxanthomonas sp. G3 are two of the few bacterial groups that can produce these biosurfactants. The research focuses on biosurfactant stability testing produced by the F1 bacteria and the G3 in a variety of temperature conditions, pH and salinity. Effectiveness of oil recovery is observed through the simulation of MEOR technology with the use of direct bacteria and biosurfactants produced by the bacteria. Bacillus sp. F1 and Pseudoxanthomonas sp. G3 are optimally grown at a temperature of 50 º C with an agitation of 125 rpm in the medium Stone Mineral Salt Solution (SMSS) with the addition of 0.1% (w/v) yeast extract and 2% (v/v) crude oil. The stability test of both biosurfactants extracts using the experimental design of Minitab software uses Box-Behnken model design. Stability testing is carried out at temperature conditions of 40 º C, 80 º C and 120 º C; pH 2, 7 and 12 as well as salinity conditions of 2%, 6% and 10%. The stability of biosurfactants is measured based on the index of Emulsification after being homogenized with crude oil and is incubation for 24 hours (E24). The simulation of the utilization of direct microorganisms and biosurfactants in MEOR technology is done by sand-pack column method using silica sand 50 mesh size. Based on the results obtained biosurfactant of Bacillus sp. has optimum stability in temperature conditions of 91.71 º C, pH 4.53 and salinity 3.21%. While the biosurfactants of Pseudoxanthomonas sp. have optimum stability in temperature conditions of 46.46 º C, pH 7.55 and 10% salinity. The results of the sand-pack column indicated that the positive control of the surfactant Triton-X gives the highest increase in oil acquisition of 18.69% and then followed by Biosurfactant F1 17.35%, biosurfactant G3 14.04%, of the G3 10.56% bacteria, the bacteria F1 10.50% Based on these results can be concluded both biosurfactants have good stability and potentially to improve the acquisition of petroleum in MEOR technology.