INDUCTION OF ARTIFICIAL AGARWOOD IN GYRINOPS VERSTEEGII SEEDLING

• Main Author: Junita, Eliana
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/47030
• Institution: Institut Teknologi Bandung

Agarwood is resin that accumulates in wood tissue as defense responses to physical/chemical/biological stresses. One of the agarwood-producing plants is Gyrinops versteegii, but its potential is still not fully utilized. The purpose of this study was to evaluate physical procedures which consisted of injury, induced by endophytic bacteria and fungi, and chemical treatments by salicylic acid as an induction agent for agarwood in G. versteegii seedling. The stems of G. versteegii was cut as long as 2 cm and inoculated by bacteria (Bacillus sp., or Alcaligenes faecalis), fungi (Fusarium solani or Paecilomyces formosus), salicylic acid and compared with healthy and injured seedling. The histochemical showed that the main group of agarwood compounds, terpen, accumulate in included phloem and xylem rays. The result of TLC analysis showed some compound had a retention index similar with commercial agarwood oil. The GC-MS analysis showed that the main compound of agarwood is chromon and was only found in plant that inoculated by bacteria and fungi. Although, composition is mixture of sesquiterpenes and detected only from F. solani. It can be concluded that biological and chemical agents can induce agarwood resin in G. versteegii seedling, but F. solani is the best agent for producing artificial agarwood.