ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITY OF ETHYL ACETATE FRACTION AND VOLATILE OIL FROM RED GINGER RHIZOME (ZINGIBER OFFICINALE ROSCOE VAR. SUNTI VAL) AS ANTI- ATHEROSCLEROTIC AGENT CANDIDATES
Atherosclerosis is an inflammatory disease that last for a long time with unseen progress. In the previous research, the assumption on red ginger as anti atherosclerosis agent is strengthened by lipid profile improvement compared to simvastatin. This study aimed to discover the active fractions f...
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| Format: | Theses |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/47078 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Atherosclerosis is an inflammatory disease that last for a long time with unseen
progress. In the previous research, the assumption on red ginger as anti
atherosclerosis agent is strengthened by lipid profile improvement compared to
simvastatin. This study aimed to discover the active fractions from red ginger
rhizomes to be developed further as anti-atherosclerosis agents. The selections are
based on anti-inflammatory and antioxidant activities. The anti-inflammatory
activity is assessed by red blood cell membrane stabilization method. While
antioxidant activities are assessed by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) also cupric ion
reducing antioxidant capacity (CUPRAC) method. The results showed that 200
µg/ml acetic salicylic acid (77.32 ± 9.97%), 400 µg/ml red ginger rhizomes
ethanol extract (42.60 ± 0.87%), 200 and 400 µg/ml ethyl acetate fraction (43.02
± 8.12% and 52.11 ± 0.30%) also 100, 200 and 400 µg/ml volatile oil (15.72 ±
17.80%; 34.46 ± 11.52%, and 53.99 ± 16.84%) exhibited hemolytic inhibitory
activity that statistically significant compared to controls. Antioxidant activity
measured by DPPH, ABTS and CUPRAC method revealed red ginger rhizomes
ethyl acetate fraction had the lowest IC50 (23.25 ± 10.74 µg/ml; 27.36 ± 6.43
µg/ml; and 15.17 ± 0.60 µg/ml). Blois classification indicated that ethanol extract
and ethyl acetate fraction of red ginger rhizomes are potent antioxidants. While
red ginger rhizomes essential oils classified as medium antioxidant by DPPH and
CUPRAC method, as well as weak antioxidant by ABTS method. In conclusion,
ethyl acetate fraction and red ginger rhizomes essential oil are prime candidates
for further development as anti-atherosclerotic agents.
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