EXPLORATION OF PROTEIN CONTENT IN SNAIL (ACHATINA FULICA) SLIME AS NATURAL SKIN AGING PREVENTION

• Main Author: Dwi Larasati, Rizky
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/47229
• Institution: Institut Teknologi Bandung

Snail Achatina fulica is one of the most extensively studied snails due to its economical, ecological and exploration in medical field. Snail slime is mainly composed of allantoin, glycolic acid, collagen, and elastin. Collagen and elastin are the most important protein involved in the process of skin aging prevention. This work is aimed to explore and characterize the protein presence in snail slime of Achatina fulica for promising candidate protein to slow the skin aging process. Snail slime was collected by manual stimulation by dredging the slime from snail body. The protein in snail slime was then characterized with SDS PAGE, and quantified using Lowry method, as well as the protein chromatogram analysis. Based on SDS PAGE, it was found bands with the size of 184.95; 74.69; 65.54; 51.08; 38.12, 17.31 and 15.86 kDa. The protein content of snail slime was 127.94 µg/mL. The chromatogram analysis using Sirius red/fast green showed that snail slime was positive for collagen, whereas Verhoeff-Van Goeven staining showed the positive sign of elastin. Snail slime showed 78.63% inhibition of collagenase and 70.34% inhibition of elastase. In vivo activity test by observation in the form of pinch test, wrinkle scores and microscopic observation showed potential activity as anti skin aging.