KELAINAN KROMOSOM PADA EMBRIO SAPI HASIL MATURASI DAN FERTILISASI SECARA IN VITRO

• Main Author: B.Samuel, Sandra
• Format: Theses
• Language: Indonesia
• Subjects: Ilmu hayati ; Biologi
• Online Access: https://digilib.itb.ac.id/gdl/view/4725
• Institution: Institut Teknologi Bandung

<b>Abstract:<p align=\"justify\"> <br /> <br /> Experiment has been carried out to study chromosome abnormalities in bovine embryos produced from in vitro maturation and fertilization of oocytes. The aim of this experiment was to analyze the relationship between the ability of early embryonic development and the incidence of chromosomal abnormalities at various stages of development. Bovine oocytes (from Ongole strain). were matured for 22-24 hours in medium 199 supplemented with 20% FBS and incubated at 39°C in 5% CO2 and 95% air. After in vitro maturation, the eggs were inseminated for 18 hours. The embryos produced were cultured for 5-7 days, and at 12 hours interval, the number of embryos reaching a developmental stage were recorded. Chromosome preparation and analysis, namely total number, morphology and sister chromatid exchange (SCE), were performed on embryos of stage 2, 4, and 8. The results revealed that there were no differences between adult and embryonic chromosome morphology. The autosomes were telocentric and sex chromosomes were submetacentric. The frequency of SCE on embryonic chromosomes were higher compared to the adult, but were not significantly different. Abnormal total number of chromosomes was one of the chromosomal abnormalities which could be detected. From the 44.4% total embryos carrying chromosome number abnormalities, most were polyploidy, besides haploidy and aneuploidy, which all were lethal to the embryos. The incidence of embryos carrying chromosomal abnormalities were increased parallel with the increasing cleavage stage of embryonic development. The failure of embryonic cleavage were also increased. It appears that there is a strong correlation between the increasing incidence of cleavage failure and the chromosomal abnormalities, which are the major possible causes of decreasing embryo viability.<p align=\"justify\"> <br /> <br />