(ALPHA) TOCOPHEROL ACCUMULATION IN CELL SUSPENSION CULTURE JATROPHA CURCAS AND JATROPHA GOSSYPIIFOLIA FROM EAST NUSA TENGGARA

• Main Author: Helsiana Mata, Meri
• Format: Theses
• Language: Indonesia
• Subjects: Ilmu hayati ; Biologi
• Online Access: https://digilib.itb.ac.id/gdl/view/47336
• Institution: Institut Teknologi Bandung

Jatropha curcas Linn. and Jatropha gossypiifolia Linn. are types of medicinal plant because they have bioactive compounds such as phenolic acids, flavonoids, saponins, tannins, alkaloids, coumarins, lignin, phenols, steroids, and terpenoids contained in leaves, bark, roots, seeds, fruits, and sap. In addition to these bioactive compounds founded other compounds, namely tocopherols which are known to have antioxidant activity and can be used to treat several diseases. Therefore, this study aims to measure ?-tocopherol in cell culture from J. curcas and J. gossypiifolia. Friable callus was induced using Murashige and Skoog (MS) and Vitamin Gamborg medium with 1.35 x 10-5 2,4-Dichlorophenoxyacetate (2,4-D) and 4,4 x 10-6 M kinetin. Cell suspension cultures were produced by a friable callus subculture in ½ MS medium with the addition of 1.35x10-5 2.4-D and 4.4 x 10-6 M Kinetin and sucrose 30 g / L. Measurement of tocopherol content is turned on at 0; 3; 6; 9; 12; 15; 18 days using the High Performance Liquid Chromatography (HPLC) method using the mobile phase of methanol:air (95:5). The results showed that callus formation from J. curcas explants and J. gossypiifolia began to be observed after explants were incubated for one week. The friable callus formation was shown by J. curcas. The accumulation of ?-tocopherol in the sixth day J. curcas cell suspension was 0.13%. In the culture medium, ?-tocopherol was detected on thebninth day. Whereas ?-tocopherol in J. gossypiifolia cell suspension culture was detected at the beginning of culture and then decreased and increased again until the ninth day. The ?-tocopherol content on the ninth day was 0.23%. After the ninth day, the ?-tocopherol content start to decrease. In the culture medium, ?-tocopherol was found from the beginning of the culture and percentage increased on the ninth day to reach a maximum 0.12% on the twelve day. The levels of ?-tocopherol in J. curcas and J. gossypiifolia were highest at roots of 0.12% and leaves of 0.27% respectively. Based on the result obtained, it can be concluded that the highest ?-tocopherol was found in the leaves of J. gossipiifolya for cell suspension cultures and managed to grow favorably in ½ MS medium and ?-tocopherol can accumulate in cell suspension cultures.