THE EFFECT OF THE EXTRACT OF AVICENNIA ALBA (BLUME) LEAVES ON THE GROWTH OF SHRIMP PATHOGENIC BACTERIA AEROMONAS SOBRIA (CHESTER) <BR />STAINER AND VIBRIO PARAHAEMOLYTICUS FUJINO ET AL.

• Main Author: Mulyaningsih (NIM : 20695501), Sri
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/4736
• Institution: Institut Teknologi Bandung

<b>Abstract :</b><p align=\"justify\">A study on the effects of extracts of Avicennia alba leaves on the growth of shrimp pathogenic bacteria Aeromonas sobria and Vibrio parahaemolyticus has been done. Avicennia alba leaves were fractionally extracted by using n-hexane, methylene chloride and ethanol solvents. Assay of the effect of the extract on growth of bacteria has been carried out with agar diffusion method. The parameter was growth inhibition zone measured after 24 hours incubation time. Toxicity test of the extract on 14th post larva stage of shrimp (Penaeus monodon Fab.) has been done by using APHA (1995) method. Observation has been carried out on mortality of 14 di post larva stage of shrimp within 96 hours observation time. The experiment used were Complex Random Design (CRD) and Least Significant Difference (LSD) with 95 % confidence level. The result of the preliminary experiment showed that only the methylene chloride extract nhibited the growth of bacteria. The methylene chloride extract at the concentration of 0; 1; 2; 3; 4 and 5 % (w/v) were tested on Aeromonas sobria and Vibrio parahaemolyticus bacteria. The methylene chloride extract at the concentration of 0,00010; 0,0018; 0,0032; 0,0056 and 0,0100 % (w/v) were tested on 14th post larva stage shrimp. The result showed that 1% (w/v) methylene chloride extract started to inhibit the growth of Aeromonas sobria and Vibrio parahaemolyticus at 1 % (w/v) with the inhibition zone of 25,791 mm² and 39,489 mm²respectively. The result of the toxicity test of the methylene chloride extract on shrimp showed that the LC_50-96 hours value was 0,0092 % (w/v).