MIKROPROPAGASI ANGGREK KELINCI (DENDROBIUM ANTENNATUM LINDL.)

• Main Author: Hanafi R, Salsabil
• Format: Final Project
• Language: Indonesia
• Subjects: Ilmu hayati ; Biologi
• Online Access: https://digilib.itb.ac.id/gdl/view/47370
• Institution: Institut Teknologi Bandung

Dendrobium antennatum Lindl. is a cut flower with fragrant and long lasting flower. As a commercial plant, a micropropagation method is needed to produce a lot of plantlet. This study aims to observe the effect of 6-benzylaminopurine (BAP) concentration and coconut water addition in D. antennatum shoot multiplication and plantlet formation. This study was separated into two steps: initiation in liquid medium and formation of shoot and root in semi-solid medium. Shoot bud with 0.5 – 1 cm length was used as explant. First, explant was cultured in liquid VW medium supplemented with 20 g/L sucrose, 5 mg/L BAP, and 15% (v/v) coconut water. Then explant was transferred to solid VW medium supplemented with 20 g/L sucrose, 0,6% gelrite, BAP (0, 5, or 10 mg/L), 15% (v/v) coconut water, and 250 mg/L active carbon. Explants cultured in semi-solid medium were subcultured every 30 days. This study shows that explant only swelled in liquid medium. Explant in semi-solid medium formed new shoots that can further developed into plantlet by forming root. New shoots in explant came from axillary bud or protocorm-like bodies (Plb) that differentiated into shoot. Formation of axillary shoot was first observed on 30th day in VW0, VW0CW, and VW5CW. Formation of Plb was first observed on 15th day in all medium. Protocorm-like bodies differentiation into shoot was first observed on 30th day in all medium except VW0. On the 60th day, explant in VW5CW medium produced most shoots with average of 4,474 ± 4,018 shoots per explant. Plantlet formation was first observed on 30th day in VW0CW and VW5CW medium. On the 60th day explant in VW0CW medium produce the most plantlet with average of 0,218 ± 0,248 plantlet per explant. Based on that result we conclude that VW5CW is the best medium for shoot multiplication and VW0CW is the best medium for plantlet regeneration.