SYNTHESIS OF 3-APTES-MODIFIED SILICA FOR COLUMN GRAVITY CHROMATOGRAPHY PARTICLE FOR SUGAR SEPARATION

• Main Author: Faustine Hartawidjaja, Gabriele
• Format: Final Project
• Language: Indonesia
• Subjects: Kimia
• Online Access: https://digilib.itb.ac.id/gdl/view/47510
• Institution: Institut Teknologi Bandung

Sugar is one of the many compounds present in foods and beverages. Sugar is difficult to be separated due to its complexity. Separation processes would be required to determine the sugar composition in samples. One of the methods that could be used is gravity column chromatography using amine group-modified silica for particle columns. The step in preparing this column is activating the silica with piranha solution to convert the siloxane functional group into active silanol functional group. Afterwards, the product is then reacted with 3-(Aminopropyl)triethoxysilane (APTES) in toluene solvent. In this experiment, three different methods were used: shaking; magnetic stirring; and microwave synthesizing to support the reaction. DRS FT-IR spectrum showed that the most efficient method was by microwave synthesizer. The modified silica was then reacted with triethylamine to deactivate the remaining silanol groups. Every stage starting from activation to deactivation was tested using DRS FT-IR method. The spectrum showed that there were new peaks with wavelength values of 800-950 cm-1 (Si-C peak); 1000-1350 cm- 1 (C-N peak); and 1550-1650 cm-1 (N-H peak). These peaks existed inside 3-APTES spectrum itself, so it could be concluded that silica modification using 3-APTES was successful. The modified silica was then packed into a gravity column chromatography using wet methods with water as the eluent. The separation of glucose and maltose has not yet been achieved. However, the concentration ratio between the two sugar fractions from this experiment showed that a separation has occurred.