AMINE-MODIFIED BIOSILICA CYCLOTELLA STRIATA TBI AS AN ADSORPTION MATRIX FOR HUMAN SERUM ALBUMIN

• Main Author: Wulansari, Indah
• Format: Theses
• Language: Indonesia
• Subjects: Kimia
• Online Access: https://digilib.itb.ac.id/gdl/view/47723
• Institution: Institut Teknologi Bandung

The diatom cell wall of Cyclotella striata TBI is an abundant and renewable source of nanoporous biosilica. These nanoporous biosilica can be utilized as adsorption matrix for human serum albumin (HSA) contained in blood plasma. Adjusting the surface charge of nanoporous biosilica to become positively charged through chemical modification can improve its performance as a matrix. The aim of this research is to change the surface charge of nanoporous biosilica obtained from C. striata TBI by adding amine groups and propyl arms. Stages of this research included seeding of C. striata TBI, cultivation of C. striata TBI in a large-scale, isolation of biosilica C. striata TBI from its biomass by nitric acid leaching treatment followed by calcination, modification of biosilica with 3- aminopropyl-trimethoxysilane (APTMS), and filtration of HSA as a plasma protein model using biosilica as matrix. The results showed that the density of C. striata TBI cells were increased from 560,000 to 1,670,000 mL?1 cells during the seeding period. Biomass TBI C. striata is harvested from culture with a productivity of 125.57 mg of biomass L?1 day?1. Biosilica was successfully purified from the biomass of C. striata TBI with a productivity of 4.19 mg of biosilica L?1 day?1.The FTIR spectrum of modified biosilica shows new absorption peaks at wave numbers 1600, 2800, and 2900 cm ?1, which are the peak vibrations of bending ?NH2 groups, symmetrical stretching vibrations of the ?CH2 group and asymmetric stretching vibrations of the ?CH2 group. The EDS spectrum also shows the presence of the element nitrogen (11.63%) in the modified C. striata TBI biosilica. This results shows that the biosilica has been successfully modified with APTMS. To determine the adsorption capacity, biosilica of C. striata TBI is used to adsorb HSA. The maximum adsorption capacity of non-modified biosilica is 48.7 mg HSA g?1, while the adsorption of APTMS-modified biosilica is 102.9 mg HSA g?1. The efficiency of HSA adsorption of modified biosilica was 99.3%. Increased capacity and efficiency of HSA adsorption in modified biosilica C. striata TBI indicate the presence of chemical adsorption due to interactions between HSA and ?NH2 groups on modified biosilica surfaces. These results suggest that the amine-modified biosilica C. striata TBI is a cost-effective, renewable, and efficient for nanofiltration of blood plasma protein