THE INFLUNCE OF FIELD CAPACITY AND INOCULATION OF <BR />INCEPTISOL SOIL FUNGUS IN BIODEGRADATION OF <BR />HERBICIDE 2,4-DICHLOROPHENOXYACETIC ACID (2,4-D)

• Main Author: Adison Fuah (NIM : 25396016), Saduartha
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/4834
• Institution: Institut Teknologi Bandung

<b>Abstract :</b><p align=\"justify\">The title of this research is \"The Influnce Of Field Capacity and Inoculation Of Inceptisol Soil Fungus Of Biodegradation Of Herbicide 2,4-Dichlorophenoxyacetic Acid (2,4-D)\". Experiment being done in this research was the arrangement of soil humidity (on water holding capacity of 50%, 75%, and 100%) which was combined with an isolate type of soil fungus (Aspergillus sp. and Penecillium sp.). The objectives of this research were to understand the effect of interaction between water holding capacity treatment and the type of inceptisol soil fungus isolates in the biodegradation process of herbicide 2,4-D and to obtain combination between water holding capacity treatment and the best inceptisol soil fungus isolate in speeding up biodegradation process of herbicide 2,4-D. This research consisted of three phases, which were: (i) isolation and identification of inceptisol soil fungus, (ii) experiment of inceptisol soil fungus\'s endurance on herbicide 2,4D, and (in) biodegradation of herbicide 2,4-1) by inceptisol soil fungus. Once herbicide touches soil, it will find a media that is heterogeneous and will interact in various forms. The use of herbicide can cause an obstruction of growth and activity of soil microorganisms which in turn the herbicide\'s persistency in soil will be longer in time. Thus, an inactivation of herbicide is very important because a herbicide molecule is made to be non-fitotoxic. The latter is done by involving a process of degradation of the herbicide molecule. In this research, the ability of microorganisms (soil fungus, in particular) to do a biodegradation process of herbicide 2,4-1) was observed. The type of soil being experimented in this research was inceptisol which was taken from the area of Jatinangor-Sumedang, West Java. To obtain the isolates of each soil, OAES method was used. Pure cultivation was made from two types of isolates that were considered dominant of inceptisol soil, which were later being used on the phase of experiment of inceptisol soil fungus\'s endurance on herbicide 2,4-D and the experiment of biodegradation of herbicide 2,4-D. The experiments of isolation, identification, and soil fungus\'s endurance on herbicide 2,4-D were conducted in the Microbiology Laboratory, Environmental Engineering Department, ITB Bandung. As of the observation of herbicide 2,4-D residue was conducted in the Laboratory of Analytic Chemistry, ITB Bandung, while the pot experiment was being done at PAU\'s green house, ITB Bandung. The result of the isolation showed that the amount of fungus per gram of soil was 13,260 cell/gr. of soil. While the result of the identification showed that the largest amount of a colony of fungus found in inceptisol soil was Aspergillus sp. at 12,300 cell/gr. of soil (93%), followed by Penicillium sp. at 840 cell/gr. of soil (6%), and Mucor sp. at 120 cell/gr. of soil (1%). Furthermore, on the experiment of Aspergillus sp.\'s and Penicillium sp.\'s endurance on herbicide 2,4-D showed that Aspergillus sp. had a better endurance compared to Penicillium sp., looking at it from the fungus\'s structural development, dry weight of mycelium, and the diameter of colony. As of the experiment of biodegradation by observing the amount of herbicide residue being left in soil showed that the combination of experimenting the soil humidity on water holding capacity of 75% and a type of fungus isolate of Aspergillus sp. resulting the smallest amount of herbicide 2,4-D residue which was at 0.40 ng/gr. of soil. The above mentioned result showed that the experiment combining a water holding capacity of 75% and Aspergillus sp. was considered the best combination in the biodegradation <br /> process of herbicide 2,4-Dichlorophenoxy-acetic-acid (2,4-D). In addition, the experiment on Czapek\'s liquid medium showed that biodegradation of herbicide 2,4-D by Aspergillus sp. and Penicillium sp. resulted in the end products: Chloride, Catechol and Phenol. <br /> <br />