EXPLORATION OF GROWTH MEDIA COMPOSITION OF PLEUROTUS GIGANTEUS, LOCAL ISOLATE MUSHROOM

• Main Author: Rinia Putri, Fernanda
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/48357
• Institution: Institut Teknologi Bandung

Pleurotus giganteus is well-known as a giant oyster mushroom. This local isolate mushroom is found around Geulis Mount, Sumedang District every rainy season. This mushroom has the potential to be grown as food. This research aims to develop production technology of Pleurotus giganteus, especially for this local isolate by exploring its growth media composition. The exploration had been carried against the main substrate and its supplementation. Exploration of the main substrate was carried out by comparing the mycelium growth of mushroom on straw, coffee husk, coffee ground, and banana pseudostem waste. Result showed that there was no mycelium growth on coffee husk and banana pseudostem. However, there was mycelium growth on the coffee and straw ground around 2,51 cm and 7,62 cm, respectively. Based on this result, straw was chosen as the main substrate for optimizing supplementation step. Supplementation was done by variating flour or rice bran concentration. Concentration of 0,5%, 1%, and 1,5% was used for flour and concentration of 1%, 2%, and 3% was used for rice bran. Optimization was done by comparing the mycelium growth and biomass production of the fruiting body among different concentration. All of the results have been tested by one-way ANOVA and continued by the Fisher LSD test. The result showed that 0,5% flour at 3% rice bran concentration gave the highest mycelium growth at 2,075 ± 0,083 cm and 1,644 ± 0,394 cm, respectively. The results showed no significant difference. Subsequently, fruiting body biomass production was analyzed on the petri dish and baglog scale by measuring the fresh and dry biomass weight and also biological efficiency. For the petri dish scale, the highest result was from 1,5% flour and 2% rice bran concentration. However, the highest result of the baglog scale was from 0,5% flour and 3% rice bran variation. Nevertheless, there was not optimum on the flour and rice bran variation. Therefore, test in other concentration range to find the optimum concentration was needed.