PENETAPAN TOTAL FENOL DAN TOTAL FLAVONOID EKSTRAK JALARAN API [PYROSTEGIA VENUSTA (KER GAWL.) MIERS], AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DENGAN METODE DPPH, SERTA REVIEW KANDUNGAN KIMIA DAN AKTIVITAS FARMAKOLOGI MARGA PYROSTEGIA

• Main Author: Noviyanti Manihuruk, Grace
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/48379
• Institution: Institut Teknologi Bandung

Free radicals are atoms or molecules that have one or more unpaired electrons in their outermost orbitals and are so reactive to bind electrons in the surrounding environment. The reactive properties of free radicals can be reduced by the use of antioxidants which can be found in a variety of plants, such as flame vine (Pyrostegia venusta). This study was aimed to determine the total phenol content (TPC), total flavonoid content (TFC) extract of leaves, twigs, and flowers of the flame vine; to investigate the antioxidant activity of ethanol extracts using DPPH (2,2-diphenyl-1-picrylhydrazyl); to analyze the correlation between TPC, TFC, and AAI DPPH; and to prepare a review article regarding chemical content and pharmacological activities of Pyrostegia genus. The crude drug was extracted by the reflux method using increasing polarity solvents, namely n-hexane, ethyl acetate, and ethanol. The determination of TPC, TFC, and AAI DPPH were performed by UV-visible spectrophotometry. The correlation between TPC, TFC, and AAI DPPH was carried out using Pearson’s method. Article review was prepared by collecting and analyzing reference. The TPC of flame vine extracts in the range of 0.993–11.052 g GAE/100 g, the TFC in the range of 0.560–20.883 g QE/100 g, and the AAI DPPH in the range of 0.682–23.217. An article review was done by considering and analyzing 70 references. The highest TPC was shown by ethyl acetate flower extract (11.052 ± 0.150 g GAE/100 g), the highest TFC by ethyl acetate leaves extract (20.883 ± 0.028 g QE/100 g). The TPC and TFC had significantly positive to AAI DPPH of ethanol extracts of leaves, twigs, and flowers of the flame vine. Ethanol leaves and flower extracts were very strong antioxidant by the DPPH method. Moreover, TPC and TFC contributed to antioxidant activities of leaves, twigs, and flowers of the flame vine by the DPPH method. Based on the literature study, the Pyrostegia genus has many chemical compounds and various pharmacological activities.