INTERACTION OF BITTER MELON (MOMORDICA CHARANTIA) FRUIT EXTRACT WITH SGLT-2 INHIBITOR DRUG IN DIABETIC MOUSE MODEL

• Main Author: Zulkaida
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/48735
• Institution: Institut Teknologi Bandung

Diabetes mellitus (DM), as one of the five main causes of death in the world, is a metabolic chronic disease caused by reduction of insulin production and/or insulin receptor sensitivity. The number of DM patient in Indonesia according to Riskesdas in 2013 was 6.9% or with an absolute number around of 12 million people. It is further estimated that in 2030 the prevalence of DM in Indonesia is around 21.3 million people. Some cases were found that DM patients use herbs as an alternative drug. One of the plants oftenly used as traditional medicine for DM in Indonesia was Bitter-melon (Momordica charantia). There is possibility that the herbs are consumed in combination with conventional medicine. Therefore, the aim of this research was to determine antidiabetic activity of bitter melon extract, its mechanism of action, and the possibility of pharmacodynamic interactions between one of conventional drug (dapagliflozin) with bitter melon (Momordica charantia). Antidiabetic test in mice was glucose tolerance test, alloxan induced diabetes, and insulin resistance test with fat induction. Dosage of bitter melon used for tolerance test, alloxan induction test, and insulin resistance test was 19.5; 39.5; and 78 mg / g bb mice, while for Dapagliflozin (DPZ) dose was 1 mg / gbb mice. The results of this study showed that bitter melon extract could reduce blood glucose level significantly compared to positive control at all doses tested. The mechanism of action of bitter melon extract preparations were probably by increasing of insulin, secretion insulin and insulin receptor sensitivity. The combination of bitter melon extract with SGLT-2 inhibitor drug did not change antidiabetic activity of the drug. Therefore, this combination did not categorize as a harmful interaction.