PENGARUH IRRADLASI SINAR ULTRAVIOLET TERHADAP VIABILITAS, KERUSAKAN INTI SEL DAN PERKEMBANGAN KULTUR PROTOPLAS KACANG HIJAU (VIGNA RADIATA (L.)WILCZEK)

• Main Author: Asih Saputri, Dwijowati
• Format: Theses
• Language: Indonesia
• Subjects: Ilmu hayati ; Biologi
• Online Access: https://digilib.itb.ac.id/gdl/view/4905
• Institution: Institut Teknologi Bandung

<b>AbstraCT :</b><p align="justify"> <br /> <br /> <br /> Effects of ultraviolet irradiation on viability, nucleus damage and the development of cultured protoplasts of mungbean (Vigna radiata (L) Wilczek) <br /> The effects of ultraviolet irradiation on protoplasts of mungbean have been studied to find out the effect of ultraviolet irradiation on viability, nucleus damage and development of cultured protoplasts of mungbean. It has been reported that UV irradiation could damage the cell nucleus and as a consequence it will inhibits the development of protoplasts culture. Protoplasts were isolated from the first leaves of 6 or 7 days of mungbean seedling with 6 enzyme compositions. Freshly isolated protoplasts, which were obtained from 7-days-old seedling using a combination of 1.5% cellulase RS, 1% Macerozyme R-10, 2.5 mM CaC12.2H2O and 0.4M manitol, were exposed to UV at dosage 1000, 2000, 4000, 6000 and 8000 erg/mm2, respectively. Protoplasts viability, cell wall regeneration and nucleus were observed under fluorescence microscope following FDA, Calcofluor white and DAPI staining, respectively. Cell wall regeneration was also observed using Scanning Electron Microscope (SEM). Protoplasts were immobilized in alginate beads with densities 1.105 protoplasts/ml before culturing in KM8P liquid medium. The result showed that protoplast viabilities were quite high (82,96%) in control and UV-treated protoplasts. Percentage of the nuclei damaged was increased in proportion to the given dosage. Cell wall regeneration was started at 2 h after culture and 8 h after culture protoplast surface was completely covered by cell wall material. First cell division was occurred 2 d or 4 d after culture in control and in all treatment dosages, respectively. Plating efficiency decreased along with irradiation dosages, 16% in control, 1% (1000 erg), 0,68% (2000 erg), 0,56 (4000 erg), 0,51% (6000 erg) and 0,32%(8000 erg). From these results it can be concluded that UV irradiation does not influence protoplast viability, cell wall regeneration but it can damage nucleus and inhibit the development of cultured protoplasts of mungbean.<p align="justify"> <br /> <br />