DEVELOPMENT AND VALIDATION OF AN ANALYTICAL METHOD FOR THE DETERMINATION OF PHENYLETHYL RESORCINOL WITH FIVE PRESERVATIVE IN SKIN WHITENING CREAM USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
Phenylethyl Resorcinol is a synthetic compound derived from natural lightening compound, Scotch Pine Bark. It is regarded as a reliable whitening agent and widely used in bleaching cream. In the market, those products are usually added with other compounds such as preservative to inhibit the grow...
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| Format: | Theses |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/49064 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Phenylethyl Resorcinol is a synthetic compound derived from natural lightening
compound, Scotch Pine Bark. It is regarded as a reliable whitening agent and
widely used in bleaching cream. In the market, those products are usually added
with other compounds such as preservative to inhibit the growth of
microorganisms. In order to control and ensure the safety of products in market,
an analytical method to determine Phenylethyl resorcinol is needed. The method
to analysis should be specific and selective, so it can provide correct analytical
signal in a mixture of analyte within sample, without any interaction between
analyte. For this purpose, an analytical method using High Performance Liquid
Chromatography (HPLC), UV detector 282 nm, Zorbax Eclipse Plus C18 ?m (4.6
x 150 mm) column was developed. Gradient system was used in mobile phase, at 1
- 4 minutes was used Acetonitrile-Water (30:70), at 4.1 - 15 minutes was used
Acetonitrile-Methanol-Water (40:20:40), and the flow rate was 1.0 mL/min.
Validation parameters performed were specificity, linearity, limit of detection
(LOD), limit of quantitative (LOQ), accuracy, precision, and robustness. The
result of each parameters from analysis of Phenylethyl resorcinol with addition of
five preservative matrix and other bleaching agents are, specificity gave
resolution >1.5, linearity in range 10.48-73.36 mg/mL and the regression
equation obtained was y=8175.30x-2709.61 with r = 0.9999 and Vx0 = 0.78, the
percentage of recovery was 97.46 –103.05%, the intraday and interday precision
was obtained HorRat value ?2, indicating the precision of analytical method was
already fulfill the requirement. Limit of detection (LOD) value was 0.7183 mg/L
and limit of quantification (LOQ) was 2.3943 mg/L. Those result showed that this
analytical method is selective, linear, accurate, and precise. This method can be
used for testing of Phenylethyl Resorcinol in skin whitening creams.
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