SYNTHESIS OF 3-APTES MODIFIED SILICA AS HIGH PERFORMANCE LIQUID CHROMATOGRAPHY PARTICLE COLUMN FOR SUGAR SEPARATION

• Main Author: Pratiwi, Resty
• Format: Final Project
• Language: Indonesia
• Subjects: Kimia
• Online Access: https://digilib.itb.ac.id/gdl/view/49120
• Institution: Institut Teknologi Bandung

Carbohydrates are the largest source of energy in the human body and are composed of different sugar monomers. The similarity in structure and functional groups of the sugar monomers makes the method of analyzing sugar monomers difficult. High Performance Liquid Chromatography (HPLC) is currently the most effective method for analyzing sugars based on accuracy, simplicity in sample preparation, and speed of analysis time compared to other methods. The most common column used for sugar separation in HPLC is active amine group column. The column with the active amine group consists of silica particles modified with aminopropyl functional group. In this research, four different methods have been carried out to synthesize silica from tetraethylortosilicate (TEOS) precursors in order to obtain the best synthesis method. Direct mixing of reagents with composition 10 mL H2O, 27.8 mL isopropanol, 12.5 mL TEOS, 20 mL of ammonia and 8.52 mL 3-APTES 20% (v/v) in isopropanol under stirring conditions for 6 hours and a temperature of 80oC is the most effective synthesis method. Characterization of the silica produced using FTIR and SEM. IR spectrum shows several new peaks such as peaks ?OH (3000 cm-1 and 1600 cm-1), Si?O in silanol (950 cm-1) and Si?O in siloxane (1100 cm-1, 800 cm-1 and 450 cm-1) when compared to the precursors. SEM results show the silica produced has a round shape with a size of around 800 ? 900 nm. Modification with aminopropyl has been successfully carried out with the emergence of new peaks in the FTIR spectrum, namely Si?C bonds (800 ? 950 cm-1), C?N bonds (1000 ? 1350 cm-1), primary N?H bonds (3360 ? 3450 cm-1) and C?H bonds (2980 ? 3050 cm-1). Packaging of the modified silica into an empty HPLC column indicate an increase in pump pressure over the 100 ? 300 bar range along with an increase of column length for same flow rate.