?-AMILASE INHIBITORY ACTIVITY OF SOME MEDICINAL PLANTS AND ISOLATION OF ACTIVE COMPOUND FROM SELECTED PLANT (FICUS RELIGIOSA L.)
Diabetes and its complication was major causes of death in most countries compared HIV/AIDS, tuberculosis and malaria. One approach in the treatment of diabetes is by slowing down the glucose absorption in the gastrointestinal tract, its can be achieved by inhibition of hydrolizing enzymes such a...
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| Format: | Theses |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/49158 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Diabetes and its complication was major causes of death in most countries
compared HIV/AIDS, tuberculosis and malaria. One approach in the treatment of
diabetes is by slowing down the glucose absorption in the gastrointestinal tract, its
can be achieved by inhibition of hydrolizing enzymes such as ?-amylase so the
absorption of glucose becomes slow. This research aim was to determine the ?-
amylase inhibitor activity from aqueous extract of the plant and from the selected
plants the active compound was isolated. Extraction was done by reflux method
using aquadest as a solvent. Extracts were obtained by freeze drying and tested for
?-amylase inhibitory activities. Percent inhibition value of bodhi leaves aqueous
extract at concentration 500 µg/mL was 72,43% ± 4,23 and it was selected to be
fractionated by liquid-liquid extraction using n-hexane and ethyl acetate. Extract
and fractions of bodhi leaves were tested for ?-amylase inhibitory activities
compared with acarbose. The IC50 value of aqueous extract, n-hexane, ethyl
acetate, water fraction of bodhi leaves and acarbose as reference were 282,53;
759,93; 459,58; 345,64; and 3,42 µg/mL, respectively. The ethyl acetate fraction
was separated using column chromatography (CC) with isochratic elution
(chloroform-acetone). Subfraction 122 and 123 were further separated using
sentrifugal thin layer chromatography with gradient elution from n-hexane-ethyl
acetate-methanol to obtain fraction containing compund X. It was purified using
centrifugal thin layer chromatography with isochratic elution (ethyl acetatechloroform-acetone-methanol) to obtain compound X. It characterized by specific
spraying reagents and was tested for ?-amylase inhibitory activities. Based on the
result of charactersization by specific spray reagent that compund X was a class of
coumarin and percent inhibition value of compound X at concentration 500
µg/mL was 57,34% ± 1,37.
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