ISOLATION AND IN SILICO ANALYSIS OF RECOMBINANT E2 PROTEIN FROM CHIKUNGUNYA VIRUS

• Main Author: Mutiara Ayu Wulandari, Raden
• Format: Final Project
• Language: Indonesia
• Subjects: Kimia
• Online Access: https://digilib.itb.ac.id/gdl/view/49588
• Institution: Institut Teknologi Bandung

Chikungunya is a disease caused by infection of Chikungunya virus (CHIKV). Generally, symptoms of the infection are high fever, headache, muscle pain, swollen joints, and skin rash. Symptoms often occur after 3 to 7 days from the initial bite by mosquitos infected with CHIKV. Currently, there is no vaccine to prevent Chikungunya. By studying the structure and function of the E2 protein, the discovery of the Chikungunya vaccine will be achieved in the future. Therefore, the aim of this study is to isolate and in silico analysis of the recombinant E2 protein CHIKV. Expression of the gene encoding recombinant E2 CHIKV in pET16b was successfully done using E. coli BL21 (DE3) as the host cell. The production of the E2 protein has been successfuly as well through a protein band of 44 kDa on SDS-PAGE gel as an inclusion body protein. The aliphatic index data of the E2 protein is 72.94, indicates that the E2 CHIKV protein is a hydrophobic protein. This data is consistent with the discovery of the dominant E2 protein in the form of inclusion body. The results of the in silico analysis of the E2 CHIKV protein with three other proteins with PDB codes of 3N41, 3N40, and 6JO8, show that there are several differences in amino acids, namely G49S, R80K, A88V, A95T, S125G, D136G, V186I, T243M, I248V, dan R249V. These findings may have implication in different physical, biochemical and immunogenic properties of the E2 protein CHIKV.