STUDI KANDUNGAN POLISIKLIK AROMATIK HIDROKARBON (PAH) DALAM SEDIMEN DENGAN METODE HPLC

• Main Author: Hermawan, Dadan
• Format: Theses
• Language: Indonesia
• Subjects: Kimia analitis
• Online Access: https://digilib.itb.ac.id/gdl/view/5052
• Institution: Institut Teknologi Bandung

<b></i>Abstract :</b><i><p align=\"justify\"> <br /> Environmentally, polycyclic aromatic hydrocarbon (PAH) compounds occur widely, although the abundance of them is very small. In fact, some of these compounds are carcinogenic. Therefore, a standard analytical method for monitoring the abundance of PAH in the environment is needed. In this research, the method of PAH analysis in sediment is developed. First, the sediment sample was reacted with KOH in methanol under reflux (saponification step), then, the sample was extracted by hexane. Aromatic compounds were separated by column chromatography which used alumina:gel silica (1:1) column and dichloromethane as an eluent. PAH, the result of column chromatography separation, was analyzed by HPLC technique. The use of C18 column with 25 cm length, methanol:water eluent, 1 mLJmin flow rate and UV detector at 254 nm wavelength, could separate and analyze the PAH in the sample