STUDI KOMPARATIF GEN PR1, PR2, PR3, PR4, DAN PR5 SECARA IN SILICO PADA PISANG PAHANG (MUSA ACUMINATA, AA) DAN KLUTUK WULUNG (MUSA BALBISIANA, BB) TERKAIT RESPON TERHADAP INFEKSI PENYAKIT DARAH (BLOOD DISEASE)

• Main Author: Sachisiva Anindita, Kenzie
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/50655
• Institution: Institut Teknologi Bandung

Banana (Musa spp.), is one of the most important food commodity in many tropical and subtropical countries. In recent decades banana production has been severely hindered by several diseases. Banana Blood Diseases caused by Ralstonia syzgii, has became one of the the main threats for banana production in Indonesia. An alternative to overcome the problem arising from plant disease is to develop a plant which tolerant to disease infection. It can be developed by utilizing genes which responsible defense mechanism to response pathogen infestation. Some studies reported that Pathogenesis Related (PR) proteins encode by PR gene family were accumulated in plant tissue during pathogen infection, suggesting its role in some model organism. In this study we aim to characterize some PR gene family including PR1, PR2, PR3, PR4, and PR5 in two different banana, Musa acuminata cv. Pahang (AA group) and Musa balbisiana cv. Klutuk Wulung (BB group), possessing different genomes (A and B genomes) by comparative genomic analysis. We reported that each corresponding PR genes from those species have been predicted to have the same number of exons and introns. The similarity percentage of genes ranging from 76,5-96,2% and protein > 79%. The abundance of Cis-acting regulatory elements that have a role as a light-responsive are dominant in each PR gene. The protein sequences of each PR genes shared the same domain associated to defense mechanism. The result from gene ontology search, showed that each PR gene has biological activities and molecular function in defense against invading pathogens. The Phylogenetics that Musa acuminata and Musa balbisiana taxa is closely related to each other and clustered in a big clade with other monocots species. The result of this study suggested that PR genes is important to be further investigated as potential markers in developing resistant banana against Blood Disease.