KARAKTERISASI DAN KOMPARASI PATHOGENESIS-RELATED GENE TRANSCRIPTIONAL ACTIVATOR (PTI5 & PTI6) PADA PISANG GENOM A (MUSA ACUMINATA) DAN GENOM B (MUSA BALBISIANA) SECARA IN SILICO : UPAYA PENINGKATAN KETAHANAN PISANG TERHADAP PENYAKIT LAYU FUSARIUM

• Main Author: Nurharuni, Indah
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/50976
• Institution: Institut Teknologi Bandung

Bananas are plant whose fruit is widely consumed by people in the world and Indonesia. The annual consumption of bananas reaches 100 million tonnes worldwide. A high number of bananas is defined as the commodity with the 4th largest production after rice, corn, wheat. in plants. Bananas are classified into 4 groups based on their genomic composition genome : A genome (Musa acuminata), B genome (Musa balbisiana), S genome (Musa schizocarpa), and T genome (Australimusa). Most edible bananas belong to the Eumusa (or Musa) section, the majority of these bananas originated from hybridization between M. acuminata and M. balbisiana species. Musa acuminata has the characteristic of sweet fruit and chategorized as dessert banana. On the other hand, Musa balbisiana has the characteristic of thick leaves, thick waxy coating, fruit containing seeds, thick fruit skin, known to be more resistant to biotic and abiotic stress. Banana production has decreased due to threats and diseases. Pathogenesis-Related Genes Transcriptional Activator (PTI5 & PTI6) is one of the transcription factors that function in disease defense With the availability of Musa acuminata Musa balbisiana genome data, this study aims to identify and compare PTI5 and PTI6 genes in B genome with the annotated model in A genome. The PTI5 and PTI6 genes in A genome were obtained from the National Center of Biotechnology Information (NCBI) and genome B from the analysis of the Basic Local Alignment Search Tool Nuclotide (BLASTN) at Banana Genome Hub.The PTI5 and PTI6 nucleotide sequences of the two genomes were compared using Pairwise Sequence Alignment to obtain the persentage of identity and similarity using the EMBOSS-Needle algorithm while the gene structure, location of exons and introns was predicted using the FGENESH + program. Amino acid sequences of PTI5 and PTI6 were identified for their domains and motifs using the CD-Search Tool and MEMEsuite program. Phylogenetic tree was constructed using PTI5 and PTI6 protein sequences from Musa acuminata, Musa balbisiana and other plants using the Neighbor-Joining method. The nucleotide sequences and protein sequences Musa acuminata and Musa balbisiana showed similar nucleotide compositions and gene structures with percentage identity 97% for PTI5 and 89% for PTI6. Protein sequences have same dan conserved domain namely APATELA2/Etylene-Responsive Factor (AP2/ERF) with YRG and RAYD motifs which have function as DNA-binding transcription factor fo Pathogenesis-Related genes.