DESIGN AND IMPLEMENTATION OF RICE QUALITY DETERMINER DNA REPLICATION DEVICE WITH PCR METHOD

• Main Author: Jonatan, Gilbert
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/50987
• Institution: Institut Teknologi Bandung

According to data from the Indonesian Ministry of Agriculture, rice is the staple food for more than 95% of Indonesia's population. However, based on data from the Central Bureau of Statistics, the quality of rice produced in Indonesia is still not good enough and does not match the price offered, so the rice produced by Indonesia is less competitive at the global level. So far, most of the quality of rice in Indonesia is still detected by physiological and physicochemical methods. This method was unable to determine some of the rice quality factors which had a direct relationship with the characteristics of the rice DNA. This can hamper rice production, especially for good quality rice. Therefore, the use of biomolecular technology developments to identify the quality of rice at the genetic level needs to be applied in Indonesia. Based on the description above, a solution is proposed in the form of a system called "DNA Detection System for Rice Quality Identifier or RIKUIDEN". The rice quality determiner DNA replication device using PCR method or PCR Device for short is part of the RIKUIDEN system. PCR Device is used to provide conditions for polymerase chain reaction. PCR Device consist of three main subsystems, namely power supply, user interface and temperature control subsystem. The power supply subsystem is used to provide power to the microprocessor and temperature actuator. The user interface subsystem received input parameters and display the reaction to the user. Finally, the temperature control subsystem is used to control the thermal block temperature according to the PCR temperature profile. PCR Device is implemented in the ESP32 microcontroller with ESP-IDF framework and uses FreeRTOS to manage task scheduling. The implementation result show that all subsystems are functioning properly.