IMMUNOPRESIPITASI ENZIM KARBAMOIL FOSFAT SINTETASE MENGGUNAKAN ANTIBODI ANTIPROTEIN CARA SALMONELLA TYPHI

• Main Author: Astuti (NIM 20599063), Winni
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/5181
• Institution: Institut Teknologi Bandung

<b></i>Abstract :</b><i><p align=\"justify\"> <br /> Carbamoyl phosphate synthethase (CPSase) from Escherichia coli K-12 consist of small subunit (CarA) and large subunit (CarB). Genes that encoded for CPSase from Escherichia coli K-12, Salmonella typhimurium LT2 and Salmonella typhi showed high homology. The purpose of this research is to investigate whether the high homology of genes followed by the same structure of CPSase. The strategy chosed was immunoprecipitation method that used CarA antibodies and antipeptide antibodies to C-terminal of CarA, and SDS PAGE analysis. Immunoprecipitation method using CarA antibodies result two bands for each bacteria, with mobility of 55 kDa and 45 kDa. Immunoprecipitation using antipeptide antibodies to C-terminal of CarA result many bands. Band of 45 kDa could be identified as CarA. Band of 55 kDa could be identified as antibodies that eluded in the release of antigen. The other bands were the coprecipitant. Based on the results, it can be suggested that structure of CPSase in S. typhimurium LT2 and S. typhi resemble to CPSase in E. colt K-12.