STUDI DOSIS RESPONS ANTARA INSEKTISIDA PROFENOFOS DENGAN AKTIVITAS ENZIM ASETILKOLINESTERASE DAN KOLINESTERASE PADA DARAH TIKUS PUTIH (RATTUS NORVEGICUS) GALUR WISTAR
<b>Abstrack</b><p align=\"justify\">Organophosphorus insectisides are extensively used in farming, housing, public health and other applications, because of their positive effects in increasing crops yields. However, organophosphorus insectisides also cause toxic effect,...
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| Format: | Theses |
| Language: | Indonesia |
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| Online Access: | https://digilib.itb.ac.id/gdl/view/5187 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | <b>Abstrack</b><p align=\"justify\">Organophosphorus insectisides are extensively used in farming, housing, public health and other applications, because of their positive effects in increasing crops yields. However, organophosphorus insectisides also cause toxic effect, due to its mechanism of action and chemical structure which affect nervous system by inhibiting acetvlcholinesterase and cholinesterase activities in blood. Consequently, uncontrolled, rapid twitching of some muscles, paralyses, convulsion, and in extreme cases, death may occur. This research is intended to study the effect of profenofos insectiside to the enzyme activity, using Rat (Rattus norvegicus) Wistar strain as testing animal by inhalation method. The activity of enzyme, the insectiside residu, the dose respons curve, and value of NOEL/.NOAEL will be determined, also safe human dose will be predicted. The activity of both enzymes were determined by spectrofotometry. The result of the experiment showed that there were decreasing activities of acetvlcholinesterase and cholinesterase enzymes with given inhalation dose of 12,2 mg/kg*day, 10,45 nng/kg*day, 6,75 mg/kg*day, 5,3 mg/kg*day, 3,87 mg/kg*day and 1,38 mg/kg*day with observed time of 6-hour, 24-hour and 48-hour. Decreasing of acetylcholinesterase enzyme in the animal tested were 8,17% - 53,07% and decreasing of cholinesterase enzyme were 9,78% - 53,99%, which were statistically significant as compared to the control group. There were strong inverse correlation between exposure dose and enzyme activity. The value of NOEL was 3,15 mg/kg*day, hence the equivalent safe human dose is predicted to be around 4,142 gram. |
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