SECONDARY METABOLITE FROM KECAPI FRUIT PEELS (SANDORICUM KOETJAPE MERR.) AND BIOACTIVITY AGAINST THYROCINE KINASE

• Main Author: Ayu Rachmadhaningtiyas, Dyah
• Format: Theses
• Language: Indonesia
• Subjects: Kimia
• Online Access: https://digilib.itb.ac.id/gdl/view/51969
• Institution: Institut Teknologi Bandung

Meliaceae contains secondary metabolite compounds from the limonoid, terpenoids, and flavonoid groups which have interesting bioactivities such as an insecticide, antifeedant, insect repellent, as well as anti-inflammatory, antioxidant and cytotoxic. Therefore, research on Meliacea is fascinating to be taken further to optimized its benefiting potentials. Although research on this plant has been carried out by other researchers, a specific research originating from Indonesian samples has not been widely carried out. Moreover, in this study, a plant tissue from the fruit peels is used, meanwhile, this part of the fruit still lacks an in-dept study. In this research, the isolation, characterization and bioactivity testing of secondary metabolites from fruit peels of Sandoricum koetjape have been carried out originated from Pandeglang, Banten. The isolation of secondary metabolites from fruit peels extract S. koetjape involves several steps; extraction, fractionation, and purification using various chromatography methods. The molecular structure of the isolated compound was determined based on spectroscopic data (MS, 1D-NMR and 2D-NMR). Three pure compounds have been isolated and identified as triterpenoids, namely brinonic acid (B1), hispidol B 3-palmitic (B2) and brionolic acid (B3). Compounds B1-B3 were evaluated as inhibitors of receptor tyrosine kinases (EGFR, HER2, HER4, IGFR, InsR, KDR, PDGFR?, and PDGFR?). The results showed only B2 that have significant activity against InsR.