ISOLATION, CHARACTERIZATION AND BIOACTIVITY EVUALUATION OF SECONDARY METABOLITES FROM ASPERGILLUS CARBONARIUS, AN ENDOPHYTIC FUNGUS FROM SEA SPONGE AAPTOS SUBERITOIDES
The discovery and development of lead compounds have shifted from terrestrial to marine organisms. Sea sponge (Porifera) is one of the most interesting organisms under the sea because of their chemical defense ability by producing various bioactive compounds. In addition, microorganisms in internal...
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| Format: | Theses |
| Language: | Indonesia |
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| Online Access: | https://digilib.itb.ac.id/gdl/view/51971 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | The discovery and development of lead compounds have shifted from terrestrial to marine organisms. Sea sponge (Porifera) is one of the most interesting organisms under the sea because of their chemical defense ability by producing various bioactive compounds. In addition, microorganisms in internal tissues of sponges (i.e endophytic fungi) are responsible for secreting various secondary metabolites in order to protect and assist their host life. Phytochemical studies showed that endophytic fungi are also promising sources of structurally novel and biologically active compounds such as cytotoxic, microbial, and viral activities. Based on those backgrounds, the objectives of this research were to isolate and characterize secondary metabolites from endophytic fungi associated with sea sponge Aaptos suberitoides and also evaluate the bioactivity of isolated compounds against murine leukemia P-388 cells and their microbial activity. Endophytic fungi from sea sponge Aaptos suberitodes were inoculated on malt extract agar media with chloramphenicol 0.4% and characterized based on the sequence of molecular rDNA ITS and reported as Aspergillus carbonarius (homology: 99%). The endophytic fungus A. carbonarius was cultivated and incubated on rice solid media for 28 days (280C) to yield 11.24 g EtOAc extract. The EtOAc extract was fractionated and further purified by using various chromatography techniques. The structures of the isolated compounds were elucidated based on spectroscopic data including 1D NMR (1H dan 13C NMR), 2D NMR (HSQC and HMBC), and mass spectroscopy. The four compounds yielded from EtOAc extract of Endophytic fungus A. carbonarius were a new isocoumarin named (R)-6-hydroxy-3-(1- hydroxypropan-2-yl)-8-methoxyisocoumarin and three known compounds were (22E,24R)-ergosta-4,6,8(14),22-tetraen-3-one, territrem B, and methyl 3,4,5- trimethoxy-2-(2-(nicotinamido)benzamido)benzoate and also two others (DN-2 and DN-3) which the structures have not been elucidated yet. |
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