THE ACTIVITY OF OXIDATIVE STRESS IN HYPERGLYCEMIA MALE RATS WITH MODERATE EXERCISE

• Main Author: Wahyuni, Tri
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/52136
• Institution: Institut Teknologi Bandung

Hyperglycemia is a condition in the body characterized by high blood glucose level, either fasting or postprandial blood glucose. High blood glucose levels in a person due to an irregular lifestyle, causing by food intake or a lack of physical activity such as exercise. Fasting blood glucose in normal people ranges from 126mg/dL, if a person is fasting and the blood glucose level exceeds that limit, mean hyperglycemia occurs, and this refers to pre-diabetes or already diabetic conditions. The hyperglycemic is believed play a role in triggering the increase of oxidative stress and causing cell damage that may lead to diabetic complications if not handled properly. The increasing production of ROS (Reactive Oxygen Species) due to hyperglycemia causing an imbalance between ROS and antioxidant in the body, leading to increasing oxidative stress conditions. The other way to overcome the increase of ROS in the body is by increasing the endogenous antioxidants. Physical exercise is one of the preventive measures recommended by WHO to prevent or delay the type 2 diabetes and its complications. In this study, physical exercise was given to male rats to treat hyperglycemia that it can reduce oxidative stress activity. This experimental was designed involving twenty-four male Wistar rats that divided into four groups: (a) Normal group (N), (b) Exercise group (T), (c), Hyperglycemia group (induced with 85 mg/kg body wiegt of alloxan injected subcutaneously – H), and (d) Hyperglycemia+Exercise group (H+T). The parameters analysed are (1) fasting blood glucose level, (2) oxidative stress status or ROS concentration by measuring the levels of: (a) MDA (malondialdehyde), (b) SOD (Superoxide Dismutase), and (c) T-SH (Total Sulfhydril Group), and (3) the hiostology of the pancreas. The experiment was carried out for three months. The blood plasma was observed at week 0 (W0), week 3rd (W3), week 6 (W6th), week 9 (W9th) and week 12th (W12). Exercise was given three days after blood was drawn at W0. Exercise was given with a duration of ±40 minutes/day, 5 days/week, for three months. Blood for the examination of fasting blood glucose (FBG) was drawn from caudal vein, while blood for ROS and antioxidants status was drawn from orbital sinus. At the end of the study, the experimental animals were killed by euthanasia, then dissected and the was isolated and prepared for histological analyses. Data were analyzed by using Kruskal Wallis test, then further test by using Mann-Whitney, and the correlation analysis by using Spearman’s Rho. We found that there was a significant difference in FBG among the groups (p<0,05). The highest fasting blood glucose (FBG) levels based on mean rank were found in the H and H+T groups. The MDA levels showed a significant difference among the groups (p<0,05) with the mean rank showing that the highest MDA was in group H, while based on time of observation, the highest MDA was at W0 and W6. There was not significant different of SOD and Total-SH levels among the groups (p>0,05) with the highest SOD levels in the N group and the highest Total SH levels in the HT group. While based on the time of observation, we found that the highest SOD level were in W9 and W12, while Total-SH were found in 0W and 12W. The correlation analyses showed that blood glucose levels of MDA and Total-SH had a weak relation, positive, and significant relation (p<0,05). While the correlation analyses between blood glucose and SOD had negative correlation (p<0,05).