OOCYTE HISTOLOGY AND VITELLOGENIN PROTEIN CONCENTRATION OF BONYLIP BARB (OSTEOCHILUS VITTATUS VALENCIENNES, 1842) AFTER INDUCTION OF 17-? ESTRADIOL
Bonylip Barb (Osteochilus vittatus) is an Indonesian fish endemic and has the potential to be developed as a cultured fish that is profitable from an economic, production and environmental sustainability perspective. Increasing the production and quality of Bonylip Barb eggs can be done by administe...
Saved in:
| Main Author: | |
|---|---|
| Format: | Theses |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/52259 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Bonylip Barb (Osteochilus vittatus) is an Indonesian fish endemic and has the potential to be developed as a cultured fish that is profitable from an economic, production and environmental sustainability perspective. Increasing the production and quality of Bonylip Barb eggs can be done by administering the 17-? estradiol hormone (E2). This study aims to determine the effect of 17-? estradiol hormone administration on oocyte development, egg quality, and the concentration of Vitellogenin protein in the blood plasma of Bonylip Barb (Osteochilus vittatus). A total of 60 mature female Bonylip Barb were divided into 5 groups, namely: 1. Natural control group (K0), 2. Control group which was injected with hormone solvent / olive oil (K1), 3. Treatment group 1, injected with E2 dose of 105 ?g/ kg bw (P1), 4. Treatment group 2, injected E2 with a dose of 210 ?g/ kg (P2), and 5. Treatment group 3, injected E2 with a dose of 420 ?g/ kg bw (P3). To homogenize the condition of the gonads, the nilem fish are first injected with Ovaprim and then spawned with stripping. One week after spawning, the fish were injected with E2 intraperitonial, then kept for 43 days. Gonad and blood samples were taken on day 1, day 15, day 29, and day 43 after the E2 injection was given. Body weight and gonad weight were weighed to calculate the value of Gonadosomatic Index (IGS). The number of eggs is counted to calculate fecundity. The gonads were made histological incisions stained with Hematoxylin Eosin (HE) which was used to observe oocyte development and measurement of oocyte diameter. Blood plasma was analyzed by ELISA method to measure the concentration of Vtg protein in plasma. The data were analyzed statistically with the homogeneity, normality, ANOVA, Tukey test, and T test. The results showed that there was an increase in the IGS value and egg diameter in line with the observation time which was significantly different between the control and treatment groups, and different in the fecundity value, especially in the group. P2 and P3. The IGS value shows the correlation between treatment and time of observation. There are 4 stages of Bonylip Barb oocytes during observation namely primary growth stage, cortical alveolus stage, vitellogenesis, and mature stage. Oocyte development was found to be different between the control group and the treatment group, where the development of oocyte in the treatment group (P2 and P3) was faster than the control group. The concentration of Vtg protein in blood plasma increased on day 15 to day 29 then decreased on day 43 in almost all treatment groups and was significantly different in the P2 group compared to other treatment groups. We conclude that the addition of 17-? Estradiol Hormone as much as 210 ?g / kg BW (P2) had the best effect in accelerating the development of nilem fish oocytes, improving egg quality as well as increasing the concentration of vitellogenin protein in blood plasma of Bonylip Barb (Osteochilus vittatus).
|
|---|