ACUTE TOXICITY TEST OF TWO PLANTS FROM TACCA GENUS USING ZEBRAFISH (DANIO RERIO) EMBRYOS AS A MODEL

• Main Author: Agus, Wahyuni
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/52986
• Institution: Institut Teknologi Bandung

Tacca is a genus of several species that are reported to have potential as a new therapeutic agent with toxic properties for several cells. Jalawure and gadung tikus are two species from the Tacca genus that can be found in Indonesia. The aim of this study was to determine the level of acute toxicity of jalawure and gadung tikus by using zebrafish (Danio rerio) embryos as a model. Sample was extracted by maceration using ethanol 96%. The acute toxicity test was carried out by using Fish Embryo acute Toxicity (FET) test protocol No. 236, Organisation for Economic Co-Operation and Development (OECD). 96 hours of static exposure using the negative control group (medium embryo), the test solution group (extract) and the positive control group (3,4-dichloroaniline 4 µg/mL). The result of acute toxicity of extract against zebrafish embryos demonstated that LC50 of jalawure leaf extract 26.06 ± 0.31 µg/mL, jalawure stem extract LC50 251.52 ± 6.15 µg/mL, jalawure corm extract 463.24 ± 9.68 µg/mL, gadung tikus leaf extract 15.04 ± 0.15 µg/mL, gadung tikus stem extract 263.73 ± 6.58 µg/mL, and gadung tikus corm extract 17.71 ± 0.12 µg/mL. Jalawure leaf extract, gadung tikus leaf extract, and gadung tikus corm extract have medium toxicity levels.