EFFECT OF BIOSURFACTANT F7 ON ENTEROCOCCUS FAECALIS BIOFILM IN ROOT CANAL OF INCISOR TOOTH

• Main Author: Mentari Putri, Destaya
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/53739
• Institution: Institut Teknologi Bandung

Infection in the root canal is caused by pulp infection. Root canal treatment is used to treat infection in the root canal. Root canal treatment failure can be caused by microorganisms. The most commonly found microorganisms in root canal treatment failure is Enterococcus faecalis. Enterococcus faecalis has the ability to form biofilm in root canal. Sodium hypochlorite (NaOCl) has the ability to eradicate the Enterococcus faecalis biofilm but has high toxicity and high surface tension. Biosurfactant can reduce surface tension and has lower toxicity. Biosurfactant F7 has the ability to eliminate the smear layer in root canal and has potential to used as antibiofilm agents. This study aims to determine the value of minimum inhibitory concentration (MIC), minimum inhibitory biofilm concentration (MBIC), and minimum biofilm eradication concentration for 50% eradication (MBEC50). In addition, test of biofilm eradication activity on tooth root was also carried out. MIC and test of biofilm eradication activity on tooth root was determined by the total plate count (TPC) method. MBIC and MBEC50 were determined by weight reduction of the wet biofilm. The biosurfactant F7 concentration used for MIC and MBIC were 25 ppm, 50 ppm, and 100 ppm. Meanwhile, biosurfactant F7 concentration used for MBEC50 were 250 ppm, 500 ppm, and 1000 ppm. Results from treatment with biosurfactant F7 were compared with NaOCl 2.5%. Biofilm eradication activity on tooth root used biosurfactant F7 500 ppm, NaOCl 2.5%, and combination of biosurfactant F7 500 ppm with NaOCl 2.5%. The results of this study showed that inhibition percentage of NaOCl 2.5% higher than biosurfactant F7 with the percentage of 99.7%, while the inhibition percentage of biosurfactant F7 is 28.61%. Biosurfactant F7 was able to inhibit biofilm with the inhibition percentage 17.64%, while the inhibition percentage of NaOCl 2.5% was 47.05%. Biosurfactant F7 was able to eradicate biofilm with the eradication percentage was 60.95%, while the eradication percentage of NaOCl 2.5% was 43.24%. The value of MIC, MBIC, and MBEC50 respectively were 25 ppm, 50 ppm, and 1000 ppm. Biofilm eradication percentage on tooth root from biosurfactant F7, NaOCl 2.5%, and combination of biosurfactant F7 with NaOCl 2.5% respectively were 24.69%, 49.2%, and 61.46%. It can be concluded, that biosurfactant F7 has the ability to inhibit and eradicate biofilm in root canal.