PURIFICATION OF STEVIOSIDE FROM STEVIA REBAUDIANA (BERTONI) LEAVES EXTRACT USING POLYMETHACRYLATE MACROPOROUS RESIN
Stevioside is a diterpenoid glycoside from Stevia plant that is used as an alternative sweetener in existence today. The non-caloric properties of this compound are used broadly as a dietary-sweetener for diabetic patients. The isolation method of this compound has been developed but encountered...
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| Format: | Theses |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/53758 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Stevioside is a diterpenoid glycoside from Stevia plant that is used as an alternative
sweetener in existence today. The non-caloric properties of this compound are used
broadly as a dietary-sweetener for diabetic patients. The isolation method of this
compound has been developed but encountered an obstacle when laboratory
method is apply in industrial scale production. In the present study, isolation of
stevioside from etanolic Stevia rebaudiana (Bertoni) leaves extract was developed
using a polymethacrylate macroporous resin. Study static adsorption/desorption
dan adsorption isothermic of resin had been conducted by shaking stevioside
solution dan resin mix in Erlenmeyer at room temperature. Some solution sample
was collected from the mix then determined the stevioside content using highperformance liquid chromatography. The adsorption ratio dan capacity of the resin
to stevioside are 152.21 mg/g dry resin dan 71.91% on a stevioside solution with
purity of >60%, respectively. On the extract solution, the adsorption ratio dan
capacity of the resin to stevioside showed 191.57 mg/g dry resin dan 65.07%,
respectively. Kinetic data for both solutions obeyed the pseudo-second-order
kinetic model. Adsorption isothermic data of extract solution showed a good
correlation coefficient of Langmuir equation with dimensionless separation factor
(RL) obtained 0.140. The value of RL was in the range of 0 < RL < 1 that concluded
the adsorption of stevioside in the polymethacrylate resin is favorable. The
adsorption stevioside into the resin occured easily due to the 1/n value in the
Freundlich equation is between 0.1 dan 0.5 (0.1802). Column packed with resin to
perform dynamic adsorpstion dan desorption tests of stevioside into resin.
Purification of stevioside using polymethacrilate macroporous resin with gradient
elution using etanol (0-70% (v/v)), the content of stevioside increased from 26.10%
to 83.82% (w/w).
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