MELANOGENESIS INHIBITOR ACTIVITY OF SEVERAL MEDICINAL PLANTS USING ZEBRAFISH (DANIO RERIO) EMBRYO AS AN ANIMAL MODEL

• Main Author: Azanti Putri, Refsya
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/54371
• Institution: Institut Teknologi Bandung

Background and purpose: Melanin is a darkening pigment secreted by melanocytes found in the basal layer of epidermis. This pigment has an important function in the protection of human skin from the harmful effects of UV light radiation and scavenging of free radicals. Current skin whitening agents have weak activity and cause adverse effects such as skin irritation and pigment depigmentation. The utilization of medicinal herb which has an antimelanogenic activity is believed to be a solution of these problems. This study aims to find the plant extracts that have an inhibitory effect on melanin production and safety with a wide range of non-toxic concentrations on zebrafish embryos. Methods: This research used seven plant species such as Curcuma longa Val.; Curcuma xanthorrhiza Roxb.; Curcuma heyneana Val.; Allium sativum L.; Allium cepa L.; Camellia sinensis L.; and Garcinia mangostana L. The extraction method in this research was maceration using methanol as the solvent. Antimelanogenesis evaluation was done with several modification from the previous publications. Result: The result shows that extracts of Curcuma xanthorrhiza and Curcuma heyneana are toxic to zebrafish embryos in all the tested concentration (12,5- 500 ppm). The five left extracts were found to inhibit the melanin production which was classified as moderate activity (10-49%). Interestingly, tea leaves extract shows a potent and non-toxic melanin inhibition activity compare with other tested extracts (IC50 121,26 ppm).