ISOLASI SENYAWA FLAVONOID DARI DAUN JATI EMAS TECTONA GRANDIS DENGAN METODE FERMENTASI

• Main Author: Maryanty, Yanti
• Format: Theses
• Language: Indonesia
• Subjects: Kimia
• Online Access: https://digilib.itb.ac.id/gdl/view/5472
• Institution: Institut Teknologi Bandung

<b>Abstract :</b><p align=\"justify\"> <br /> <br /> Tectona grandis is one of well-known woody plants that has been cultivated due to its high economic value and has a function as a critical land rehabilitator. As a part of Angiospermae, it also contains flavonoid. Flavonoid has biological activities as an antioxidant by acting as a free radical scavenger. Quercetine and rutine are part of flavonoid com unds that have been found in Angiospermae. Flavonoid compounds are isolated by liquid extraction. At this research, isolation of flavonoid compounds from Tectona grandis was conducted by submerged and solid state fermentation using Aspergillus niger. Continue extraction (soxhletation method) was used as a comparison method.<p align=\"justify\"> <br /> <br /> Optimal growth of Aspergillus niger in 100% Tectona grandis leaves medium to produced flavonoid by submerged fermentation was 48 hour and pH 3.75. The yield of flavonoid produced by this process was 0.39%. While in the solid state fermentation process, optimum conditions were pH 3.8, agitated at 75 rpm and incubated two weeks fermentation time. Semi-quantitative analysis was conducted using HPLC (Waters 2487 with column C18 length of 25 x 4.6 mm and diameter 0.48 mm) and eluted with methanol: water (67.5: 32.5) with a flow rate of 1 ml min-1. At this condition rutine and quercetine retention time were detected at 3,32 mV.min-1 and 5,52 mV.min-1 respectively. HPLC results showed that the percentages of total flavonoid, rutin and quersetin were 0.13%, 0.0001%, 0.002% for submerged fermentation; 0.59%, 0.12%, 0.1% for solid state fermentation; 8.62%, 0.022% and 0.023% for continue extraction (soxhletation) respectively.<p align=\"justify\"> <br /> <br /> These results showed that although soxhletation method can isolate total flavonoid 14.5 times higher than solid state fermentation, the percentages of rutin and quercetin isolated by solid state fermentation were 5.4 and 4.3 time higher than soxhletation method.<p align=\"justify\">