SEPARATION OF TWO OBJECTS BASED ON SIZE DIFFERENCE USING HYDRODINAMIC PRINCIPLE WHICH IS USED IN LEUKOCYTE SEPARATION USING MICROFLUID

• Main Author: Zaahid Alkindi, Muhammad
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/54895
• Institution: Institut Teknologi Bandung

Blood is one of the most important elements in every living thing's life due to its function in distributing oxygen and nutrients thoroughly inside the body to maintain the stability of the body. Blood also plays a big role as the first defense against bacteria, viruses, or any unknown object that entered the body. Blood comprises blood plasma and blood cells (which are comprised of 3 other parts, erythrocytes, leukocytes, and platelets). Blood separation is rather hard to undergo due to the composition of erythrocytes are extremely massive compared to any other components. One of the solutions to cope with this problem is using microfluid. Microfluid is a science that studies fluid flow behavior on a micro-scale using particular physical properties of those cells. One of the methods used in microfluid is the hydrodynamic principle, which utilizes the Hagen-Poiseuille equation as the main factor in the separation of the fluid and 2 objects which are differed by the size difference using the concept of Ohm’s law and Kirchoff’s law. Apparently, we can apply this concept in separating two different-sized objects with fluid as the carrier. In this final project, we are looking for the accuracy of separation between 2 distinguished objects and the final volume contained in the outlet. To retrieve the data, we were using 2 different treatments. We will place the objects preemptively in the vessel for the first treatment. For the second treatment, we will introduce the fluid through the channel firstly before placing the object in the vessel. The results of these experiments tell us that for the first treatment, we were successfully delivering 15 of 20 bigger objects to the outlet, with 64.1 mL of fluid contained in the outlet (4.133% of the total volume of the fluid). In comparison, we were successfully driving 12 of 20 bigger objects to the outlet for the second treatment, with 228 mL of fluid contained in the outlet (6.998% of the total volume). After scrutinizing the result of the previous experiments, new questions have arisen. For that reason, we perform a new experiment to learn the spread pattern of the balls delivered by the fluid through the channel, which will answer the proneness of which channel is likely to be contained with each of the objects. For the experiment to determine the spread of smaller objects (bullet toy gun with d = 6mm), the objects were prone to be contained in the left side of the channel from the viewpoint of inlet A. It occurred due to the unevenness of the channel (it's likely to be weighted to the left side). After several experiments, this behavior gradually changed, because of the deformability of the channel. For the experiment to determine the spread of the larger objects (Styrofoam ball with d = 10mm), the objects were likely to be contained in the outlet. The results concurred with what we have expected, that for those objects larger than 7.941 mm (virtual width that entered the side channel) will not be allowed to go to the side channels, instead flowing out to the outlet.