MEROPENEM IMPRINTED POLYMER AS SELECTIVE SORBENT FOR SOLID PHASE EXTRACTION METHOD FOR MEROPENEM ANALYSIS IN HUMAN BLOOD PLASMA

• Main Author: Sirumapea, Lasmaryna
• Format: Dissertations
• Language: Indonesia
• Subjects: Kimia
• Online Access: https://digilib.itb.ac.id/gdl/view/54986
• Institution: Institut Teknologi Bandung

Meropenem is antibiotic of carbapenem group, which has beta-lactam ring on its main structure. Meropenem is given to treat many kinds of infection caused by bacteria, and also to treat fever that followed by the decrease of leucosyt. This kind of antibiotic is not over the counter medicine, it is given only on doctor’s presciption. Meropenem is an antibiotic that has high sensitivity on many kinds of microbes. The role of analytical methods is very important in the success of drug analysis in biological matrices. Sensitivity of analytical method has to be high, because a drug usually present in the plasma in a small amount. Analytical method has to be selective due to the existence of other compounds, which can interfere the analysis. Solid phase extraction is one type of sample preparation that uses sorbent. Until now, sorbent for solid phase extraction of meropenem is still a conventional sorbent and has not been made with a molecular imprinting technique. Meropenem is an antidiabetic drug with a betalactam ring in its main structure. Methacrylic acid (MAA) and acrylamide (ACR) monomer has never been reported as a functional monomer in molecular imprinted polymer. The aim of this study was to have a solid phase extraction sorbent for meropenem by using molecular imprinted techniques using meropenem as a template molecule or commonly known as molecular imprinted polymer solid phase extraction (MI- SPE). The sorbent was used to increase the separation efficiency of meropenem in biological matrices. The research was begun with polymerization stage using the bulk method and then ended with application of the sorbent for separation of compounds in human blood plasm. Synthesis was carried out under heating of 70ºC; bulk polymerization method was choosen because its simplicity. Methacrylic acid, acrylamide and itaconic acid were applied as functional monomer. EGDMA, BPO, and DMSO were the crosslinker, initiator and porogen solvent respectively. Characterization of MI-SPE sorbent was done through adsorption condition’s optimization, selectivity test, determination of MIP adsorption capacity, repeatability test and evaluation of breaktrough volume. Physical characterization of MI-SPE sorbent was carried out by using fourier transform infra red (FTIR) and scanning electron microscope (SEM). The research was ended with application of MI-SPE for extraction of meropenem in human blood plasm. Adsorption capacities of MeIP1 and MeIP2 were determinated by using Freundlich adsorption isoterm It was found that adsorption capacity of MeIP1 and MeIP2 were 51,963 mg/g and 55,017 respectively. Physical characterization using FTIR showed hydrogen bonding interactions between a monomer-template from C=O group wavenumber shifting before and after template extraction. FTIR investigation showed specific peaks of MeIP1 at 950 cm-1 and 1018,95 cm-1 that indicates C-N and C-H bond in betalactam ring. Spesific peak of betalactam ring found in FTIP spectrum of MeIP2 at 1049,28 cm-1 indicated the wagging and twisting vibration of C-H . Soxletation method was the best extraction method that gave best adsorption capacity compared to the other method applied. Optimation of adsorption conditions of MeIP1 and MeIP2 through the column were carried out with the amount of sorbent = 50 mg, with the cartridge SPE of maximum capacity of 1 mL. Feed solution was the analyte solution in phosphate buffer solution at pH = 3. The optimum condition were : methanol as the conditioning solvent, acetonitrile : water 95 : 5 v/v as the washing solvent, and methanol : acetic acid 8 : 2 (v/v) was choosen as the elution solvent. Imprinting factors of MeIP1 and MeIP2 were then calculated for the amoxicillin an cefadroxil as analogue. The recovery of this parameter were 1,35 and 2,012 to cefadroxil and amoxicillin respectively. The retention volume (volume breakthrough = VB) was also observed, and the VB point was 8 ml (MeIP1) and 6 ml (MeIP2) For the application of blood plasm, the recovery was determined. It is found that MeIP2 gave the better recovery (83,86%) than MeIP1 (78,52%). The reuseability of adsorption was also determined for MeIP2, i.e in the range of 75,902 % - 86,648%.