FABRICATION AND MODIFICATION OF GRAPHENE AND SILVER NANOPARTICLES MODIFIED SCREEN-PRINTED CARBON ELECTRODE FOR THE DETERMINATION OF RHODAMIN B

• Main Author: Eka Kartika, Andi
• Format: Theses
• Language: Indonesia
• Subjects: Kimia
• Online Access: https://digilib.itb.ac.id/gdl/view/55105
• Institution: Institut Teknologi Bandung

Rhodamine B (RhB) is carcinogenic textile dye that is widely misused as a food colorant. Therefore, it is necessary to develop a simple and sensitive method to determine RhB. This compound is electrochemically active and could be detected using voltammetry. RhB was determined using sensor screen-printed carbon electrode (SPCE-Gra/AgNPS) which is fabricated by using screen-printing technology. Graphene (Gra) and Silver Nanoparticle (AgNPs) were used as composite to modify working electrode (SPCE-Gra/AgNPS) by using drop casting method. The morphology of modified working electrode was characterized by Scanning Electron Microscopy-Energy Dispersive X-Ray (SEM-EDX). The sensitive determination of RhB on SPCE-Gra/AgNPS in phosphate buffer solution 0,1 M pH 6 was observed by SWV technique. The evaluation of the anodic peak current, effect of the optimum concentration of graphene, pH of the analyte solution, and the scan rate were optimized in this study. The result show that the anodic peak current of RhB increased using SPCE-Gra/AgNPS in comparison to the bare SPCE. Calibration curve exhibited linier ranges from 2 – 100 µM and limit of detection was 1,94 µM. Furthermore, SPCE-Gra/NPA showed good reproducibility and quite accurate recovery percentage of 105,4%. The GSs-AgNPs sensor demonstrated a selective detection of RhB in the presence of common interfering compounds present in the food samples, including sucrose, monosodium glutamic, and sodium benzoic. When the sensor was used for the determination of RhB in an actual food sample, similar results were shown as suggested by UV-Vis spectroscopy analysis. Hence, the fabricated sensor can be applied for the determination of RhB in food samples.