SECONDARY METABOLITES FROM LEAVES OF CRYPTOCARYA NITENS AND THEIR CYTOTOXICITY

• Main Author: Erlinda Octarina, Risa
• Format: Theses
• Language: Indonesia
• Subjects: Kimia
• Online Access: https://digilib.itb.ac.id/gdl/view/55375
• Institution: Institut Teknologi Bandung

Cryptocarya is one of genus belongs to Lauraceae family which grows in tropical and subtropical regions, such as Asia, Australia, Africa and America. Indonesian people knows this plant as medang-medangan or huru. The main secondary metabolites of this genus are pyrones, flavonoids and alkaloids. Pyrones and flavonoids are two major secondary metabolites of Indonesian Cryptocarya while alkaloid is the main secondary metabolites of Australian Cryptocarya. Secondary metabolites of this genus have been reported possesing various activities, i.e anticancer, antimalarial, antiinflammation, antibacterial and antiviral. One of Indonesian Cryptocarya is Cryptocarya nitens. Previous investigation has reported that the main secondary metabolite from the leaves of this species was pyrone. According to chromatogram of Thin Layer Chromatography (TLC) of resuled fractions, they still contain potential compounds to be separated. Therefore, in this research, the continuing isolation of leaves of Cryptocarya nitens was conducted. The isolation of secondary metabolites have been carried out by several separation methods including extraction at room temperature (maceration) using acetone, chlorophyll separation with methanol - water (1:1) followed by extraction with ethyl acetate, and then separation and purification using various of chromatographic techniques, including vacuum liquid chromatography (VLC) and gravitational columns chromatography (GCC). In this continuing research, seven compounds have been isolated, six of them were identified as pyrone derivatives, namely deacetylcryptocaryalactone, (+)- (6R,2'R)-cryptocaryalactone, (+)-(6R,2'S)-cryptocaryalactone, cryptomoschatone E1, cryptofolione, and 7-styryl-2,6-dioxabicyclo-[3.3.1]nonan-3-on, along with one known flavonoid derivative, i.e. kaempferol. Four compounds, i.e. cryptomoschatone E1, cryptofolione 7-styryl-2,6-dioxabicyclo[3.3.1]nonan-3-one, and kaempferol were obtained in this continuing research. Moreover, cryptomoschatone E1 was isolated for the first time from the leaves of Cryptocarya, but it has been obtained from the bark of Cryptocarya moschata. The finding of pyrones and flavonoid from the leaves of Cryptocarya nitens supports the statement that Indonesian Cryptocarya have a tendency to produce pyrone and flavonoid compounds. Cytotoxic biassay data of isolated compounds showed that there were active compounds against murine leukemia P-388 cells which can be potential as lead compounds for anticancer.