BIODEGRALLASI KLOROLIGNIN OLEH PSEUDOMONAS PUTIDA ATCC 45491 DAN PHANEROCHAETE CHRYSOSPORIUM BKMF 1767

• Main Author: Pingkan Aditiawati
• Format: Dissertations
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/5540
• Institution: Institut Teknologi Bandung

<b> Abstrak : </b><p align="justify">Wastewater from the bleaching process in the pulp and paper industry contains a chlorinated organic compound known as chlorolignin. Chlorolignin has many damaging effects on the environment since low molecular weight of chlorolignin is toxic in nature, mutagenic, and carcinogenic, while high molecular weight chlorolignin is difficult to degrade. Physical and chemical treatments of wastewater have been conducted, but the results are not satisfactory due to the high cost required. Conventional biological treatment using full scale activated sludge and anaerobic or aerobic lagoon system are capable of removing only 19 - 66% of the chlorolignin and more toxic compounds are also produced. <br /> <br /> The research was conducted on the chlorolignin degradation by a microbiological method in which single cultures of Pseudomonas putida and Phanerochaete chrysosporium were used, after which both cultures were used consecutively. It is known that P. chrysosporium has been used to degrade lignin in the process of the color removal of Bleach Plant Effluent, nevertheless this study will attempt to increase ligninolytic activity of the microorganisms by optimalization of environmental conditions that influence the microorganisms. On the other hand, study upon P. putida is conducted in order to discover the dehalogenation and degradation capability towards chlorolignin as a substrate - as studies concerning this matter have not been done. The microorganisms had potentials to be used in the waste treatment due to their wide substrate specificity. The use of a mixed culture in a consecutive manner is expected to increase the degradation rate of chlorolignin and furthermore would result in a higher percentage of chlorolignin removal without the production of the toxic intermediate compounds. <br /> <br /> The research was performed in several stages using synthetic chlorolignin. Initially, the study was done on the degradation of chlorolignin by P. chrysosporium, then by P. putida, and in the next step a consecutive application of P. putida and P chrysosporium was conducted. The degradation of chlorolignin was done using a stirred liquid medium in a 100 mL erlenmeyer flask and